ArticleApplied and environmental microbiology2014
Metabolic shift of Escherichia coli under salt stress in the presence of glycine betaine.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
- Macromolecular crowding has opposite effects on two critical sub-steps of transcription initiation.FEBS letters · 2024Article
- Applications of genome-scale metabolic models to investigate microbial metabolic adaptations in response to genetic or environmental perturbations.Briefings in bioinformatics · 2023Review
- Understanding the Probiotic Bacterial Responses Against Various Stresses in Food Matrix and Gastrointestinal Tract: A Review.Probiotics and antimicrobial proteins · 2023Review
- About the dark corners in the gene function space of Escherichia coli remaining without illumination by scientific literature.Biology direct · 2023Article
- Shift of Choline/Betaine Pathway in RecombinantInternational journal of molecular sciences · 2022Article
- Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells.Communications biology · 2022Article
- Transcriptome analysis of L-leucine-producing Corynebacterium glutamicum under the addition of trimethylglycine.Amino acids · 2022Article
- Morphological Phenotypes, Cell Division, and Gene Expression ofMicroorganisms · 2022Article
- Haloferax litoreum sp. nov., Haloferax marinisediminis sp. nov., and Haloferax marinum sp. nov., low salt-tolerant haloarchaea isolated from seawater and sediment.Antonie van Leeuwenhoek · 2021Article
- A stress-induced block in dicarboxylate uptake and utilization inJournal of bacteriology · 2021Article
- Improving L-threonine production in Escherichia coli by elimination of transporters ProP and ProVWX.Microbial cell factories · 2021Article
- Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress.Journal of bacteriology · 2019Article
- Article
- Transcriptomic and Ectoine Analysis of HalotolerantFrontiers in microbiology · 2018Article
- Effects of sodium chloride on heat resistance, oxidative susceptibility, motility, biofilm and plaque formation of Burkholderia pseudomallei.MicrobiologyOpen · 2017Article
- Article
- Understanding the interplay of carbon and nitrogen supply for ectoines production and metabolic overflow in high density cultures of Chromohalobacter salexigens.Microbial cell factories · 2017Article
- Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen Vibrio anguillarum.Journal of microbiology (Seoul, Korea) · 2017Article
- To Modulate Survival under Secondary Stress Conditions, Listeria monocytogenes 10403S Employs RsbX To Downregulate σB Activity in the Poststress Recovery Stage or Stationary Phase.Applied and environmental microbiology · 2016Article
- Exposure of Salmonella enterica Serovar Typhimurium to Three Humectants Used in the Food Industry Induces Different Osmoadaptation Systems.Applied and environmental microbiology · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
An important area of food safety focuses on bacterial survival and growth in unfavorable environments. In order to understand how bacteria adapt to stresses other than nutrient limitation in batch cultures, we need to develop mechanistic models of intracellular regulation and metabolism under stress. We studied the growth of Escherichia coli in minimal medium with added salt and different osmoprotectants. To characterize the metabolic efficiency with a robust parameter, we identified the optical density (OD) values at the inflection points of measured "OD versus time" growth curves and described them as a function of glucose concentration. We found that the metabolic efficiency parameter did not necessarily follow the trend of decreasing specific growth rate as the salt concentration increased. In the absence of osmoprotectant, or in the presence of proline, the metabolic efficiency decreased with increasing NaCl concentration. However, in the presence of choline or glycine betaine, it increased between 2 and 4.5% NaCl before declining at 5% NaCl and above. Microarray analysis of the transcriptional network and proteomics analysis with glycine betaine in the medium indicated that between 4.5 and 5% NaCl, the metabolism switched from aerobic to fermentative pathways and that the response to osmotic stress is similar to that for oxidative stress. We conclude that, although the growth rate appeared to decrease smoothly with increasing NaCl, the metabolic strategy of cells changed abruptly at a threshold concentration of NaCl.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.