Evidence map›Paper›PMID 24858046›Full record

ArticleOncogene2015

Cooperation of Blm and Mus81 in development, fertility, genomic integrity and cancer suppression.

S El Ghamrasni, R Cardoso, M J Halaby, D Zeegers, S Harding, R Kumareswaran, T Yavorska, N Chami, A Jurisicova, O Sanchez and 4 more

Abstract read
PubMed Publisher
In one paragraph

Article in Oncogene, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Article
  6. Smoking and Male Infertility: An Evidence-Based Review.The world journal of men's health · 2015
    Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

S El Ghamrasni1] Department of Medical Biophysics, University of Toronto, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada [2] Laboratory of Biotechnology, Faculty of Sciences DHEM, University SMBA, Fez, Morocco.
R CardosoDepartment of Medical Biophysics, University of Toronto, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada.
M J HalabyDepartment of Medical Biophysics, University of Toronto, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada.
D ZeegersDepartment of Physiology, Yong Loo Lin School of Medicine and Tembusu College, National University of Singapore, Singapore, Singapore.
S HardingDepartment of Medical Biophysics, University of Toronto, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada.
R KumareswaranDepartment of Medical Biophysics, University of Toronto, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada.
T YavorskaDepartment of Obstetrics and Gynecology and Physiology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, Canada.
N ChamiLaboratory of Biotechnology, Faculty of Sciences DHEM, University SMBA, Fez, Morocco.
A JurisicovaDepartment of Obstetrics and Gynecology and Physiology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, Canada.
O SanchezDepartment of Pathology, University of Ontario Institute of Technology, Oshawa, Ontario, Canada.
M P HandeDepartment of Physiology, Yong Loo Lin School of Medicine and Tembusu College, National University of Singapore, Singapore, Singapore.
R BristowDepartment of Medical Biophysics, University of Toronto, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada.
R HakemDepartment of Medical Biophysics, University of Toronto, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada.
A HakemDepartment of Medical Biophysics, University of Toronto, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada.
Ontario Institute for Cancer Research · CAUniversity of Toronto · CANational University of Singapore · SGMount Sinai Hospital · CAUniversity of Ontario Institute of Technology · CA

Funding

Canadian Institutes of Health Research
6 · The paper itself

Abstract

BLM is a DNA helicase important for the restart of stalled replication forks and for homologous recombination (HR) repair. Mutations of BLM lead to Bloom Syndrome, a rare autosomal recessive disorder characterized by elevated levels of sister chromatid exchanges (SCEs), dwarfism, immunodeficiency, infertility and increased cancer predisposition. BLM physically interacts with MUS81, an endonuclease involved in the restart of stalled replication forks and HR repair. Herein we report that loss of Mus81 in Blm hypomorph mutant mice leads to infertility, and growth and developmental defects that are not observed in single mutants. Double mutant cells and mice were hypersensitive to Mitomycin C and γ-irradiation (IR) compared with controls and their repair of DNA double-strand breaks (DSBs) mediated by HR pathway was significantly defective, whereas their non-homologous-end-joining repair was elevated compared with controls. We also demonstrate the importance of the loss of the nuclease activity of Mus81 in the defects observed in Mus81(-/-) and double mutant cells. Exacerbated IR-induced chromosomal aberration was observed in double mutant mice and despite their reduced SCE levels, these mutants showed increased tumorigenesis risks. Our data highlight the importance of Mus81 and Blm in DNA DSB repair pathways, fertility, development and cancer.

Indexed as

AnimalsCell Transformation, NeoplasticChromosome AberrationsDNA-Binding ProteinsDNA Breaks, Double-StrandedDNA End-Joining RepairEndonucleasesGamma RaysLymphomaMiceMice, Inbred C57BLMice, KnockoutMitomycinNucleic Acid Synthesis InhibitorsRecQ HelicasesBloom syndrome proteinDNA-Binding ProteinsEndonucleasesMitomycinMus81 protein, mouseNucleic Acid Synthesis InhibitorsRecQ Helicases

Identifiers

PMID24858046
OpenAlexW2014962191

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.