Trial reportPloS one2014
Detection of Puumala hantavirus antigen in human intestine during acute hantavirus infection.
Trial report in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- Alcohol Consumption and Its Influence on the Clinical Picture of Puumala Hantavirus Infection.Viruses · 2022Article
- Immunohistochemical diagnosis of human infectious diseases: a review.Diagnostic pathology · 2022Review
- Cells of the human respiratory tract support the replication of pathogenic Old World orthohantavirus Puumala.Virology journal · 2021Article
- Acute Appendicitis Associated with Hantaan Virus Infection.The American journal of tropical medicine and hygiene · 2021Article
- Mother-to-Child Transmission of Andes Virus through Breast Milk, ChileEmerging infectious diseases · 2020Article
- Orthohantavirus Pathogenesis and Cell Tropism.Frontiers in cellular and infection microbiology · 2020Review
- Prevalence and identification of arthropod-transmitted viruses in Kassala state, Eastern Sudan.The Libyan journal of medicine · 2019Article
- Article
- Serum Markers Associated with Severity and Outcome of Hantavirus Pulmonary Syndrome.The Journal of infectious diseases · 2019Article
- Three asymptomatic animal infection models of hemorrhagic fever with renal syndrome caused by hantaviruses.PloS one · 2019Article
- Evaluation of lipase levels in patients with nephropathia epidemica--no evidence for acute pancreatitis.BMC infectious diseases · 2015Article
- Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPuumala virus (PUUV) is the most important hantavirus species in Central Europe. Nephropathia epidemica (NE), caused by PUUV, is characterized by acute renal injury (AKI) with thrombocytopenia and frequently gastrointestinal symptoms.
methods456 patients with serologically and clinically confirmed NE were investigated at time of follow-up in a single clinic. The course of the NE was investigated using medical reports. We identified patients who had endoscopy with intestinal biopsy during acute phase of NE. Histopathological, immunohistochemical and molecular analyses of the biopsies were performed.
resultsThirteen patients underwent colonoscopy or gastroscopy for abdominal pain, diarrhea, nausea and vomiting during acute phase of NE. Immunohistochemistry (IHC) revealed PUUV nucleocapsid antigen in 11 biopsies from 8 patients; 14 biopsies from 5 patients were negative for PUUV nucleocapsid antigen. IHC localized PUUV nucleocapsid antigen in endothelial cells of capillaries or larger vessels in the lamina propria. Rate of AKI was not higher and severity of AKI was not different in the PUUV-positive compared to the PUUV-negative group. All IHC positive biopsies were positive for PUUV RNA using RT-PCR. Phylogenetic reconstruction revealed clustering of all PUUV strains from this study with viruses previously detected from the South-West of Germany. Long-term outcome was favorable in both groups.
conclusionsIn patients with NE, PUUV nucleocapsid antigen and PUUV RNA was detected frequently in the intestine. This finding could explain frequent GI-symptoms in NE patients, thus demonstration of a more generalized PUUV infection. The RT-PCR was an effective and sensitive method to detect PUUV RNA in FFPE tissues. Therefore, it can be used as a diagnostic and phylogenetic approach also for archival materials. AKI was not more often present in patients with PUUV-positive IHC. This last finding should be investigated in larger numbers of patients with PUUV infection.
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