Evidence map›Paper›PMID 24857743›Full record

ArticleMethods in cell biology2014

Approaches to studying subnuclear organization and gene-nuclear pore interactions.

Defne Emel Egecioglu, Agustina D'Urso, Donna Garvey Brickner, William H Light, Jason H Brickner

Abstract read
In one paragraph

Article in Methods in cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
4.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 19 citations in OpenAlex.

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  13. Microbial cell (Graz, Austria) · 2015
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Defne Emel EgeciogluDepartment of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA.
Agustina D'UrsoDepartment of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA.
Donna Garvey BricknerDepartment of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA.
William H LightDepartment of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA.
Jason H BricknerDepartment of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA.
Northwestern University · US

Funding

CELLULAR &MOLECULAR BASIS OF DISEASE TRAINING PROGRAMT32GM008061 · NIGMS · NORTHWESTERN UNIVERSITY · PI CARTHEW, RICHARD W. · 1985 to 2022
$17.1M
Role of DNA localization in regulating transcriptionR01GM080484 · NIGMS · NORTHWESTERN UNIVERSITY · PI BRICKNER, JASON HAYS · 2007 to 2019
$3.6M
NIGMS NIH HHS 2T32GM008061-31NIGMS NIH HHS GM 080484NIGMS NIH HHS R01 GM080484NIGMS NIH HHS T32 GM008061
6 · The paper itself

Abstract

Many genes in budding yeast Saccharomyces cerevisiae associate with the nuclear pore complex (NPC), which impacts their location within the nucleus and their transcriptional regulation. To understand how eukaryotic genomes are spatially organized, we have used multiple approaches for analyzing the localization and transcription of genes. We have used these approaches to study the role of DNA elements in targeting genomic loci to the NPC and how these interactions regulate transcription, chromatin structure and the spatial organization of the yeast genome. These studies combine yeast molecular genetics with live-cell microscopy and biochemistry. Here, we present detailed protocols for these cytological and molecular approaches.

Indexed as

Cell Line, TumorChromatin ImmunoprecipitationDNA, FungalGenetic VariationGenetic VectorsGenome, FungalGreen Fluorescent ProteinsHeLa CellsHumansLac OperonMicroscopy, ConfocalNuclear PorePlasmidsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription, GeneticDNA, FungalGreen Fluorescent ProteinsSaccharomyces cerevisiae ProteinsURA3 protein, S cerevisiaeChromatinChromosomeConfocal microscopyNuclear architectureNuclear pore complex

Identifiers

PMID24857743
PMCPMC4697751
OpenAlexW2416060510

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.