Evidence map›Paper›PMID 24857739›Full record

ArticleMethods in cell biology2014

NPC mimics: probing the mechanism of nucleocytoplasmic transport.

Tijana Jovanovic-Talisman, Brian T Chait, Michael P Rout

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Tijana Jovanovic-TalismanDepartment of Molecular Medicine, Beckman Research Institute of the City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Brian T ChaitLaboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, USA.
Michael P RoutLaboratory of Cellular and Structural Biology, The Rockefeller University, New York, USA.

Funding

TR&D Project 4. The Imaging Stage: Multiscale Spatiotemporal Modeling of Macromolecular Systems in Cellular NeighborhoodsP41GM109824 · NIGMS · ROCKEFELLER UNIVERSITY · PI ROUT, MICHAEL P · 2014 to 2023
$18.8M
National Resource for Mass Spectrom of Biological MacromoleculesP41GM103314 · NIGMS · ROCKEFELLER UNIVERSITY · PI CHAIT, BRIAN T · 2012 to 2017
$9.9M
Virtual Gating Machines for Protein PurificationR01GM071329 · NIGMS · ROCKEFELLER UNIVERSITY · PI ROUT, MICHAEL P · 2004 to 2013
$4.6M
NIGMS NIH HHS P41 GM103314NIGMS NIH HHS P41 GM109824NIGMS NIH HHS R01 GM071329
6 · The paper itself

Abstract

In vitro mimics of cellular machines have been recently engineered and utilized to investigate processes within cells. These devices can provide novel insights into biological mechanisms and have the potential to improve biotechnological processes such as separation. In particular, several devices have been designed to mimic translocation through nuclear pore complexes (NPCs). We describe here the fabrication of a biomimetic NPC using nanoporous filters lined with FG-repeats of proteins that create a selectivity barrier. We show the utility of this nanoselective filter as a testbed for the investigation of nucleocytoplasmic transport and demonstrate that this device closely reproduces key features of trafficking through the NPC.

Indexed as

Active Transport, Cell NucleusBacterial Proteinsbeta KaryopherinsBiomimetic MaterialsGlutathione TransferaseGoldLipid BilayersLuminescent ProteinsMicroscopy, ConfocalModels, BiologicalMuramidaseNanoporesNuclear PoreNuclear Pore Complex ProteinsNucleocytoplasmic Transport ProteinsPerforinBacterial Proteinsbeta KaryopherinsGlutathione TransferaseGoldLipid BilayersLuminescent ProteinsMuramidaseNuclear Pore Complex ProteinsNucleocytoplasmic Transport ProteinsNUTF2 protein, humanPerforinpolycarbonatePolycarboxylate CementPregnancy Proteinsran GTP-Binding ProteinThrombinyellow fluorescent protein, BacteriaFlux measurementsNanoporesNPCNPC mimicsNucleocytoplasmic transportPermeabilityTransport kinetics

Identifiers

PMID24857739
PMCPMC4272007

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.