ArticleCell2014
Molecular profiling of neurons based on connectivity.
Article in Cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed, 1 synthesis or guideline pooled it, 149 citations in OpenAlex.
- Functional genomic mechanisms of opioid action and opioid use disorder: a systematic review of animal models and human studies.Molecular psychiatry · 2023Pooled it
- Generalization of negative memories drives the development of psychological distress-related behaviors.Innovation (Cambridge (Mass.)) · 2026Article
- Risk reshapes amygdala representation of choice.bioRxiv : the preprint server for biology · 2025Article
- Disturbed engram network caused by NPTX downregulation underlies aging-related contextual fear memory deficits.Cell research · 2025Article
- Changes in nucleus accumbens core translatome accompanying incubation of cocaine craving.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2025Article
- The Formation and Function of the VTA Dopamine System.International journal of molecular sciences · 2024Review
- Neuronal and Molecular Mechanisms Underlying Chronic Pain and Depression Comorbidity in the Paraventricular Thalamus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Molecularly stratified hypothalamic astrocytes are cellular foci for obesity.Research square · 2024Article
- State and rate-of-change encoding in parallel mesoaccumbal dopamine pathways.Nature neuroscience · 2024Article
- Article
- Molecular Profiling of VGluT1 AND VGluT2 Ventral Subiculum to Nucleus Accumbens Shell Projections.Neurochemical research · 2023Article
- Genetically encoded intrabodies as high-precision tools to visualize and manipulate neuronal function.Seminars in cell & developmental biology · 2022Article
- Applications of nanobodies in brain diseases.Frontiers in immunology · 2022Review
- Virally encoded connectivity transgenic overlay RNA sequencing (VECTORseq) defines projection neurons involved in sensorimotor integration.Cell reports · 2021Article
- Neurokinin B-Expressing Neurons of the Central Extended Amygdala Mediate Inhibitory Synaptic Input onto Melanin-Concentrating Hormone Neuron Subpopulations.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- A Small Virus to Deliver Small Antibodies: New Targeted Therapies Based on AAV Delivery of Nanobodies.Microorganisms · 2021Review
- Advances in Gene Delivery Methods to Label and Modulate Activity of Upper Motor Neurons: Implications for Amyotrophic Lateral Sclerosis.Brain sciences · 2021Review
- Pain and itch processing by subpopulations of molecularly diverse spinal and trigeminal projection neurons.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.Cell metabolism · 2021Article
- Specificity in sociogenomics: Identifying causal relationships between genes and behavior.Hormones and behavior · 2021Review
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The complexity and cellular heterogeneity of neural circuitry presents a major challenge to understanding the role of discrete neural populations in controlling behavior. While neuroanatomical methods enable high-resolution mapping of neural circuitry, these approaches do not allow systematic molecular profiling of neurons based on their connectivity. Here, we report the development of an approach for molecularly profiling projective neurons. We show that ribosomes can be tagged with a camelid nanobody raised against GFP and that this system can be engineered to selectively capture translating mRNAs from neurons retrogradely labeled with GFP. Using this system, we profiled neurons projecting to the nucleus accumbens. We then used an AAV to selectively profile midbrain dopamine neurons projecting to the nucleus accumbens. By comparing the captured mRNAs from each experiment, we identified a number of markers specific to VTA dopaminergic projection neurons. The current method provides a means for profiling neurons based on their projections.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.