Evidence map›Paper›PMID 24855519›Full record

ArticleInternational journal of stem cells2009

In Vitro Differentiation and Expansion of Intrathymic T Cell Progenitors from Human Umbilical Cord Blood-Derived CD34(+) Cells.

Bo Sun, Sang-Bum Park, Ji-Won Jung, Kwang-Won Seo, Yong-Soon Lee, Kyung-Sun Kang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of stem cells, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Bo SunAdult Stem Cell Research Center, Seoul National University, Seoul, Korea ; Laboratory of Stem Cell and Tumor Biology, Seoul National University, Seoul, Korea ; Zoonotic Disease Institute, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
Sang-Bum ParkAdult Stem Cell Research Center, Seoul National University, Seoul, Korea ; Laboratory of Stem Cell and Tumor Biology, Seoul National University, Seoul, Korea.
Ji-Won JungAdult Stem Cell Research Center, Seoul National University, Seoul, Korea ; Laboratory of Stem Cell and Tumor Biology, Seoul National University, Seoul, Korea ; Zoonotic Disease Institute, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
Kwang-Won SeoAdult Stem Cell Research Center, Seoul National University, Seoul, Korea ; Laboratory of Stem Cell and Tumor Biology, Seoul National University, Seoul, Korea.
Yong-Soon LeeLaboratory of Stem Cell and Tumor Biology, Seoul National University, Seoul, Korea ; Zoonotic Disease Institute, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
Kyung-Sun KangAdult Stem Cell Research Center, Seoul National University, Seoul, Korea ; Laboratory of Stem Cell and Tumor Biology, Seoul National University, Seoul, Korea ; Zoonotic Disease Institute, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
Seoul National University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesCD4 positive cells play a central role in many lethal diseases, such as AIDS, cancer and autoimmunity diseases. CD4(-) commitment of hematopoietic stem cells involved in T cell lineage, monocyte and dendritic cells development. In this study, we showed that CD4 commitment out of thymus which may happen when hematopoietic cells undergo monocyte, dendritic cells or even earlier T cell progenitor differentiation. METHODS AND

resultsafter culturing in our medium for more than five weeks, CD4(-)CD34(+) fraction, isolated from human umbilical cord blood, decreased to 1%. However, the fraction expressing CD4 went up to 86.5%. After CD4(+) cells were cultured in methylcellulose-based CFU medium, about 40 colonies/2×10(4) cells could developed. An activation of notch-1 pathway in the freshly isolated CD34(+) cells and up-regulation of PI3K/JNK/c-Myc pathway may provide an explanation for the differentiation and proliferation of CD4(+) cells from CD34(+) hematopoietic stem cells respectively.

conclusionsACD4(+) enriched population was obtained after highly purified CD34(+) cells, isolated from human cord blood, underwent long term culture in a feeder layer-free culturing system. Colonigenic ability was maintained in the population of CD4(+) cells. This finding will be a benefit for the studies on the cell therapy for immune dysfunctions.

Indexed as

CD34+ cellsDifferentiationIntrathymic T cell progenitorsPropagationUmbilical cord blood

Identifiers

PMID24855519
PMCPMC4021793
OpenAlexW2063124931

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.