ArticleInternational journal of nanomedicine2014
Aneuploidogenic effects and DNA oxidation induced in vitro by differently sized gold nanoparticles.
Article in International journal of nanomedicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Preliminary Study on Lutetium-177 and Gold Nanoparticles: Apoptosis and Radiation Enhancement in Hepatic Cancer Cell Line.Current issues in molecular biology · 2024Article
- Gold Nanoparticles (AuNPs)-Toxicity, Safety and Green Synthesis: A Critical Review.International journal of molecular sciences · 2024Review
- Bionanofactory for green synthesis of collagen nanoparticles, characterization, optimization, in-vitro and in-vivo anticancer activities.Scientific reports · 2024Article
- Assessment of the potential cerebellar toxicity of gold nanoparticles on the structure and function of adult male albino rats.Bioscience reports · 2023Article
- Cytotoxicity Evaluation of Photosensitizer-Conjugated Hexagonal Upconverting Nanoparticles.Nanomaterials (Basel, Switzerland) · 2023Article
- Gold Nanoparticles Induced Size Dependent Cytotoxicity on Human Alveolar Adenocarcinoma Cells by Inhibiting the Ubiquitin Proteasome System.Pharmaceutics · 2023Article
- An updated overview of some factors that influence the biological effects of nanoparticles.Frontiers in bioengineering and biotechnology · 2023Review
- Nanomaterial genotoxicity evaluation using the high-throughput p53-binding protein 1 (53BP1) assay.PloS one · 2023Article
- The use of nanoparticles in the treatment of infectious diseases and cancer, dental applications and tissue regeneration: a review.Frontiers in medical technology · 2023Review
- Evaluating nanobiomaterial-induced DNA strand breaks using the alkaline comet assay.Drug delivery and translational research · 2022Article
- Assessing Genotoxicity of Ten Different Engineered Nanomaterials by the Novel Semi-Automated FADU Assay and the Alkaline Comet Assay.Nanomaterials (Basel, Switzerland) · 2022Article
- Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations.International journal of molecular sciences · 2021Review
- In Vitro Assessment of the Genotoxic Potential of Pristine Graphene Platelets.Nanomaterials (Basel, Switzerland) · 2021Article
- Toxicity of Nanoparticles in Biomedical Application: Nanotoxicology.Journal of toxicology · 2021Review
- Reactive Oxygen Species-Related Nanoparticle Toxicity in the Biomedical Field.Nanoscale research letters · 2020Review
- Size, Surface Functionalization, and Genotoxicity of Gold Nanoparticles In Vitro.Nanomaterials (Basel, Switzerland) · 2020Article
- Article
- Murine exposure to gold nanoparticles during early pregnancy promotes abortion by inhibiting ectodermal differentiation.Molecular medicine (Cambridge, Mass.) · 2018Article
- Calcium-dependent cyto- and genotoxicity of nickel metal and nickel oxide nanoparticles in human lung cells.Particle and fibre toxicology · 2018Article
- Gold Nanoparticle-Induced Cell Death and Potential Applications in Nanomedicine.International journal of molecular sciences · 2018Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gold nanoparticles (Au NPs) are used in many fields, including biomedical applications; however, no conclusive information on their potential cytotoxicity and genotoxicity mechanisms is available. For this reason, experiments in human primary lymphocytes and murine macrophages (Raw264.7) were performed exposing cells to spherical citrate-capped Au NPs with two different nominal diameters (5 nm and 15 nm). The proliferative activity, mitotic, apoptotic, and necrotic markers, as well as chromosomal damage were assessed by the cytokinesis-block micronucleus cytome assay. Fluorescence in situ hybridization with human and murine pancentromeric probes was applied to distinguish between clastogenic and aneuploidogenic effects. Our results indicate that 5 nm and 15 nm Au NPs are able to inhibit cell proliferation by apoptosis and to induce chromosomal damage, in particular chromosome mis-segregation. DNA strand breaks were detected by comet assay, and the modified protocol using endonuclease-III and formamidopyrimidine-DNA glycosylase restriction enzymes showed that pyrimidines and purines were oxidatively damaged by Au NPs. Moreover, we show a size-independent correlation between the cytotoxicity of Au NPs and their tested mass concentration or absolute number, and genotoxic effects which were more severe for Au NP 15 nm compared to Au NP 5 nm. Results indicate that apoptosis, aneuploidy, and DNA oxidation play a pivotal role in the cytotoxicity and genotoxicity exerted by Au NPs in our cell models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.