Evidence map›Paper›PMID 24855277›Full record

ArticleStem cells translational medicine2014

Directed differentiation of human induced pluripotent stem cells toward bone and cartilage: in vitro versus in vivo assays.

Matthew D Phillips, Sergei A Kuznetsov, Natasha Cherman, Kyeyoon Park, Kevin G Chen, Britney N McClendon, Rebecca S Hamilton, Ronald D G McKay, Josh G Chenoweth, Barbara S Mallon and 1 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Research progress on the application of framework nucleic acid in bone regeneration.Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2021
    Article
  11. Article
  12. Review
  13. Conventional and Emerging Markers in Stem Cell Isolation and Characterization.Advances in experimental medicine and biology · 2021
    Review
  14. Article
  15. Article
  16. Review
  17. Article
  18. Direct Reprogramming of Mouse Fibroblasts into Functional Osteoblasts.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2020
    Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Matthew D PhillipsCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Sergei A KuznetsovCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Natasha ChermanCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Kyeyoon ParkCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Kevin G ChenCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Britney N McClendonCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Rebecca S HamiltonCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Ronald D G McKayCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Josh G ChenowethCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Barbara S MallonCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Pamela G RobeyCraniofacial and Skeletal Diseases Branch, Division of Intramural Research, National Institute of Dental and Craniofacial Research, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; The NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, NIH, U.S. Department of Health and Human Services, Bethesda, Maryland, USA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA probey@dir.nidcr.nih.gov.

Funding

The role of stem cells in skeletal health and diseaseZIADE000380 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI ROBEY, PAMELA G · 2009 to 2025
$30.6M
Human pluripotent stem cellsZICNS003008 · NINDS · NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE · PI ROBEY, PAMELA · 2009 to 2025
$21.2M
Intramural NIH HHS
6 · The paper itself

Abstract

The ability to differentiate induced pluripotent stem cells (iPSCs) into committed skeletal progenitors could allow for an unlimited autologous supply of such cells for therapeutic uses; therefore, we attempted to create novel bone-forming cells from human iPSCs using lines from two distinct tissue sources and methods of differentiation that we previously devised for osteogenic differentiation of human embryonic stem cells, and as suggested by other publications. The resulting cells were assayed using in vitro methods, and the results were compared with those obtained from in vivo transplantation assays. Our results show that true bone was formed in vivo by derivatives of several iPSC lines, but that the successful cell lines and differentiation methodologies were not predicted by the results of the in vitro assays. In addition, bone was formed equally well from iPSCs originating from skin or bone marrow stromal cells (also known as bone marrow-derived mesenchymal stem cells), suggesting that the iPSCs did not retain a "memory" of their previous life. Furthermore, one of the iPSC-derived cell lines formed verifiable cartilage in vivo, which likewise was not predicted by in vitro assays.

Indexed as

Cell DifferentiationChondrogenesisOsteogenesisAgedAged, 80 and overAnimalsBiological AssayCell LineCellular ReprogrammingChondrocytesFemaleGene Expression Regulation, DevelopmentalHumansInduced Pluripotent Stem CellsMaleMesenchymal Stem CellsBoneChondrogenesisInduced pluripotent stem cellsOsteoblastTransplantation

Identifiers

PMID24855277
PMCPMC4073820

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.