ArticleTissue engineering. Part A2014
Positive selection for bone morphogenetic protein receptor type-IB promotes differentiation and specification of human adipose-derived stromal cells toward an osteogenic lineage.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Optimization and Implication of Adipose-Derived Stem Cells in Craniofacial Bone Regeneration and Repair.Bioengineering (Basel, Switzerland) · 2024Review
- Long-Term Changes in Adipose Tissue in the Newly Formed Bone Induced by Recombinant Human BMP-2 In Vivo.Biomimetics (Basel, Switzerland) · 2023Article
- Musculoskeletal tissue engineering: Adipose derived stromal cell implementation for the treatment of osteoarthritis.Biomaterials · 2022Review
- Bone morphogenetic protein 2 is a depot-specific regulator of human adipogenesis.International journal of obesity (2005) · 2019Article
- Discussion: Adipose-Derived Stem Cells and Ceiling Culture-Derived Preadipocytes Cultured from Subcutaneous Fat Tissue Differ in Their Epigenetic Characteristics and Osteogenic Potential.Plastic and reconstructive surgery · 2019Article
- Relative contributions of adipose-resident CD146NPJ Regenerative medicine · 2019Article
- Human Adipose-Derived Stromal Cell Isolation Methods and Use in Osteogenic and Adipogenic In Vivo Applications.Current protocols in stem cell biology · 2017Article
- A Review of Cell-Based Strategies for Soft Tissue Reconstruction.Tissue engineering. Part B, Reviews · 2017Review
- Isolation of CD248-expressing stromal vascular fraction for targeted improvement of wound healing.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2017Article
- Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model.Journal of visualized experiments : JoVE · 2017Article
- A Selective Cell Population from Dermis Strengthens Bone Regeneration.Stem cells translational medicine · 2017Article
- Biomimetics of Bone Implants: The Regenerative Road.BioResearch open access · 2017Review
- A Novel Method of Human Adipose-Derived Stem Cell Isolation with Resultant Increased Cell Yield.Plastic and reconstructive surgery · 2016Article
- A Review of the Clinical Side Effects of Bone Morphogenetic Protein-2.Tissue engineering. Part B, Reviews · 2016Review
- Microfluidic single-cell transcriptional analysis rationally identifies novel surface marker profiles to enhance cell-based therapies.Nature communications · 2016Article
- Enrichment of Adipose-Derived Stromal Cells for BMPR1A Facilitates Enhanced Adipogenesis.Tissue engineering. Part A · 2016Article
- Mesenchymal Stem Cells and Cutaneous Wound Healing: Current Evidence and Future Potential.Stem cells international · 2015Review
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13 authors.
Funding
Abstract
backgroundAdipose tissue represents an abundant and easily accessible source of multipotent cells that may serve as an excellent building block for tissue engineering. However, adipose-derived stromal cells (ASCs) are a heterogeneous group and subpopulations may be identified with enhanced osteogenic potential.
methodsHuman ASC subpopulations were prospectively isolated based on expression of bone morphogenetic protein receptor type-IB (BMPR-IB). Unsorted, BMPR-IB(+), and BMPR-IB(-) cells were analyzed for their osteogenic capacity through histological staining and gene expression. To evaluate their in vivo osteogenic potential, critical-sized calvarial defects were created in immunocompromised mice and treated with unsorted, BMPR-IB(+), or BMPR-IB(-) cells. Healing was assessed using microcomputed tomography and pentachrome staining of specimens at 8 weeks.
resultsIncreased osteogenic differentiation was noted in the BMPR-IB(+) subpopulation, as demonstrated by alkaline phosphatase staining at day 7 and extracellular matrix mineralization with Alizarin red staining at day 14. This was also associated with increased expression for osteocalcin, a late marker of osteogenesis. Radiographic analysis demonstrated significantly enhanced healing of critical-sized calvarial defects treated with BMPR-IB(+) ASCs compared with unsorted or BMPR-IB(-) cells. This was confirmed through pentachrome staining, which revealed more robust bone regeneration in the BMPR-IB(+) group.
conclusionBMPR-IB(+) human ASCs have an enhanced ability to form bone both in vitro and in vivo. These data suggest that positive selection for BMPR-IB(+) and manipulation of the BMP pathway in these cells may yield a highly osteogenic subpopulation of cells for bone tissue engineering.
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