ArticleInvestigative ophthalmology & visual science2014
Vimentin knockdown decreases corneal opacity.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Update on the Physiopathology of Keratoconus.Medical sciences (Basel, Switzerland) · 2026Review
- Putative Self-Organizing Human Corneal Organoids Recapitulate Human Corneal Architecture and Cellular Diversity.Bioengineering (Basel, Switzerland) · 2026Article
- Fabrication of a 3D Corneal Model Using Collagen Bioink and Human Corneal Stromal Cells.Journal of functional biomaterials · 2025Article
- The Role of Vimentin in Human Corneal Fibroblast Spreading and Myofibroblast Transformation.Cells · 2024Article
- Congenital pterygium with anterior segment dysgenesis: rare ocular manifestation in Rubinstein-Taybi syndrome.BMJ case reports · 2024Article
- The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.The ocular surface · 2023Article
- Morphofunctional Properties of Corneal Stromal Cells.Bulletin of experimental biology and medicine · 2021Article
- Protective Effects of Estrogen via Nanoparticle Delivery to Attenuate Myelin Loss and Neuronal Death after Spinal Cord Injury.Neurochemical research · 2021Article
- Vimentin as a Cap of Invisibility: Proposed Role of Vimentin in Rabbit Hemorrhagic Disease Virus (RHDV) Infection.Viruses · 2021Article
- Keratocyte mechanobiology.Experimental eye research · 2020Review
- Primary cilia in corneal development and disease.Zoological research · 2020Review
- S100A4 Silencing Facilitates Corneal Wound Healing After Alkali Burns by Promoting Autophagy via Blocking the PI3K/Akt/mTOR Signaling Pathway.Investigative ophthalmology & visual science · 2020Article
- Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections.International journal of molecular sciences · 2020Review
- Identification of Common Genes and Pathways in Eight Fibrosis Diseases.Frontiers in genetics · 2020Article
- Molecular markers for corneal epithelial cells in larval vs. adult Xenopus frogs.Experimental eye research · 2019Article
- Vimentin intermediate filament assembly regulates fibroblast invasion in fibrogenic lung injury.JCI insight · 2019Article
- Desmin deficiency is not sufficient to prevent corneal fibrosis.Experimental eye research · 2019Article
- Aniridia-related keratopathy: Structural changes in naïve and transplanted corneal buttons.PloS one · 2018Article
- Effect of Isolation Technique and Location on the Phenotype of Human Corneal Stroma-Derived Cells.Stem cells international · 2017Article
- Vimentin filament organization and stress sensing depend on its single cysteine residue and zinc binding.Nature communications · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeWound induced corneal fibrosis can lead to permanent visual impairment. Keratocyte activation and differentiation play a key role in fibrosis, and vimentin, a major structural type III intermediate filament, is a required component of this process. The purpose of our study was to develop a nonviral therapeutic strategy for treating corneal fibrosis in which we targeted the knockdown of vimentin.
methodsTo determine the duration of plasmid expression in corneal keratocytes, we injected a naked plasmid expressing green fluorescent protein (GFP; pCMV-GFP) into an unwounded mouse corneal stroma. We then injected pCMV-GFP or plasmids expressing small hairpin RNA in the corneal wound injury model (full-thickness corneal incision) to evaluate opacification.
resultsGFP expression peaked between days 1 and 3 and had prominent expression for 15 days. In the corneal wound injury model, we found that the GFP-positive cells demonstrated extensive dendritic-like processes that extended to adjacent cells, whereas the vimentin knockdown model showed significantly reduced corneal opacity.
conclusionsThese findings suggest that a nonviral gene therapeutic approach has potential for treating corneal fibrosis and ultimately reducing scarring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.