Transposon-mediated targeted and specific knockdown of maternally expressed transcripts in the ascidian Ciona intestinalis.

Takako Iitsuka, Kaoru Mita, Akiko Hozumi, Mayuko Hamada, Nori Satoh, Yasunori Sasakura

Open access · goldAbstract read

In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.5field-weighted citation impact, top 29% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

piRNA-like small RNAs are responsible for the maternal-specific knockdown in the ascidian Ciona intestinalis Type A.Scientific reports · 2018
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Takako IitsukaShimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka 415-0025, Japan.

Kaoru MitaShimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka 415-0025, Japan.

Akiko HozumiShimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka 415-0025, Japan.

Mayuko HamadaMarine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.

Nori SatohMarine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.

Yasunori SasakuraShimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka 415-0025, Japan.

University of Tsukuba · JPOkinawa Institute of Science and Technology Graduate University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Maternal mRNAs play crucial roles during early embryogenesis of ascidians, but their functions are largely unknown. In this study, we developed a new method to specifically knockdown maternal mRNAs in Ciona intestinalis using transposon-mediated transgenesis. We found that GFP expression is epigenetically silenced in Ciona intestinalis oocytes and eggs, and this epigenetic silencing of GFP was used to develop the knockdown method. When the 5' upstream promoter and 5' untranslated region (UTR) of a maternal gene are used to drive GFP in eggs, the maternal gene is specifically knocked down together with GFP. The 5' UTR of the maternal gene is the major element that determines the target gene silencing. Zygotic transcription of the target gene is unaffected, suggesting that the observed phenotypes specifically reflect the maternal function of the gene. This new method can provide breakthroughs in studying the functions of maternal mRNAs.

Indexed as

Gene Silencing5' Untranslated RegionsAnimalsAnimals, Genetically ModifiedCiona intestinalisDNA MethylationDNA Transposable ElementsEgg ProteinsEmbryo, NonmammalianFemaleGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsIn Situ HybridizationRNA, MessengerRNA Stability5' Untranslated RegionsDNA Transposable ElementsEgg ProteinsGreen Fluorescent ProteinsRNA, Messenger

Identifiers

PMID24854849

DOI10.1038/srep05050

PMCPMC4031475

OpenAlexW2083069863

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.