ArticleBiomaterials2014
The potential of label-free nonlinear optical molecular microscopy to non-invasively characterize the viability of engineered human tissue constructs.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Autofluorescence lifetime of gelatin-methacrylate hydrogels is sensitive to changes in crosslinking and post-gelation pH.Frontiers in bioengineering and biotechnology · 2026Article
- Delineation of gastrointestinal tumors biopsies using a fluorescence lifetime imaging optical fiber probe.Journal of biophotonics · 2025Article
- Autofluorescence imaging of endogenous metabolic cofactors in response to cytokine stimulation of classically activated macrophages.Cancer & metabolism · 2023Article
- FLIM imaging revealed spontaneous osteogenic differentiation of stem cells on gradient pore size tissue-engineered constructs.Stem cell research & therapy · 2023Article
- Article
- A novel method for determining murine skeletal muscle fiber type using autofluorescence lifetimes.The Journal of general physiology · 2022Article
- Toxicological Analysis of Hepatocytes Using FLIM Technique: In Vitro versus Ex Vivo Models.Cells · 2021Article
- Bioengineering tools for probing intracellular events in T lymphocytes.WIREs mechanisms of disease · 2021Review
- Cells/colony motion of oral keratinocytes determined by non-invasive and quantitative measurement using optical flow predicts epithelial regenerative capacity.Scientific reports · 2021Article
- Noninvasive Optical Assessment of Implanted Tissue-Engineered Construct SuccessTissue engineering. Part C, Methods · 2021Article
- Optical Metric Assessed Engineered Tissues Over a Range of Viability States.Tissue engineering. Part C, Methods · 2019Article
- Evaluating Cell Metabolism Through Autofluorescence Imaging of NAD(P)H and FAD.Antioxidants & redox signaling · 2019Review
- Quantitative Label-Free Imaging of 3D Vascular Networks Self-Assembled in Synthetic Hydrogels.Advanced healthcare materials · 2019Article
- Noninvasive Optical Assessment of Implanted Engineered Tissues Correlates with Cytokine Secretion.Tissue engineering. Part C, Methods · 2018Article
- Quantitative, Label-Free Evaluation of Tissue-Engineered Skeletal Muscle Through Multiphoton Microscopy.Tissue engineering. Part C, Methods · 2017Article
- Development of Low-Cost Instrumentation for Single Point Autofluorescence Lifetime Measurements.Journal of fluorescence · 2017Article
- On chip two-photon metabolic imaging for drug toxicity testing.Biomicrofluidics · 2017Article
- Optical methods for quantitative and label-free sensing in living human tissues: principles, techniques, and applications.Advances in physics · 2016Article
- Non-linear optical flow cytometry using a scanned, Bessel beam light-sheet.Scientific reports · 2015Article
- Biochemical indicators of implantation success of tissue-engineered oral mucosa.Journal of dental research · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Nonlinear optical molecular imaging and quantitative analytic methods were developed to non-invasively assess the viability of tissue-engineered constructs manufactured from primary human cells. Label-free optical measures of local tissue structure and biochemistry characterized morphologic and functional differences between controls and stressed constructs. Rigorous statistical analysis accounted for variability between human patients. Fluorescence intensity-based spatial assessment and metabolic sensing differentiated controls from thermally-stressed and from metabolically-stressed constructs. Fluorescence lifetime-based sensing differentiated controls from thermally-stressed constructs. Unlike traditional histological (found to be generally reliable, but destructive) and biochemical (non-invasive, but found to be unreliable) tissue analyses, label-free optical assessments had the advantages of being both non-invasive and reliable. Thus, such optical measures could serve as reliable manufacturing release criteria for cell-based tissue-engineered constructs prior to human implantation, thereby addressing a critical regulatory need in regenerative medicine.
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Registered trials
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