ArticleScientific reports2014
Nanoliter-scale protein crystallization and screening with a microfluidic droplet robot.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Shoebill: an interpretable AlphaFold2-informed predictor of protein crystallization propensity using XGBoost.Briefings in bioinformatics · 2026Article
- Precise loading of scarce reagents on droplet microarrays.bioRxiv : the preprint server for biology · 2026Article
- Two-Layer Droplet Arrays Enable Dynamic Manipulation of Cell Microenvironment During High-Throughput Bacterial Cultivation.Small methods · 2025Article
- Transforming microfluidics for single-cell analysis with robotics and artificial intelligence.Lab on a chip · 2025Review
- Open micro-combinatorial analysis systems of crystal growth critical points of a π-conjugated molecule in ionic liquid nanoliter droplets.RSC advances · 2025Article
- A droplet robotic system enabled by electret-induced polarization on droplet.Nature communications · 2024Article
- Open microfluidics: droplet microarrays as next generation multiwell plates for high throughput screening.Lab on a chip · 2024Review
- Origin of Species before Origin of Life: The Role of Speciation in Chemical Evolution.Life (Basel, Switzerland) · 2021Review
- Room-temperature crystallography using a microfluidic protein crystal array device and its application to protein-ligand complex structure analysis.Chemical science · 2020Article
- Complete inclusion of bioactive molecules and particles in polydimethylsiloxane: a straightforward process under mild conditions.Scientific reports · 2019Article
- Counting Proteins in Single Cells with Addressable Droplet Microarrays.Journal of visualized experiments : JoVE · 2018Article
- Microfluidic chambers using fluid walls for cell biology.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A novel dual-well array chip for efficiently trapping single-cell in large isolated micro-well without complicated accessory equipment.Biomicrofluidics · 2018Article
- Nanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells.Nature communications · 2018Article
- Advances in microscale separations towards nanoproteomics applications.Journal of chromatography. A · 2017Review
- Understanding pre-mRNA splicing through crystallography.Methods (San Diego, Calif.) · 2017Review
- An open-pattern droplet-in-oil planar array for single cell analysis based on sequential inkjet printing technology.Biomicrofluidics · 2017Article
- A versatile platform for surface modification of microfluidic droplets.Lab on a chip · 2017Article
- Protein crystallization screens developed at the MRC Laboratory of Molecular Biology.Drug discovery today · 2016Review
- Vision-based Nano Robotic System for High-throughput Non-embedded Cell Cutting.Scientific reports · 2016Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large-scale screening of hundreds or even thousands of crystallization conditions while with low sample consumption is in urgent need, in current structural biology research. Here we describe a fully-automated droplet robot for nanoliter-scale crystallization screening that combines the advantages of both automated robotics technique for protein crystallization screening and the droplet-based microfluidic technique. A semi-contact dispensing method was developed to achieve flexible, programmable and reliable liquid-handling operations for nanoliter-scale protein crystallization experiments. We applied the droplet robot in large-scale screening of crystallization conditions of five soluble proteins and one membrane protein with 35-96 different crystallization conditions, study of volume effects on protein crystallization, and determination of phase diagrams of two proteins. The volume for each droplet reactor is only ca. 4-8 nL. The protein consumption significantly reduces 50-500 fold compared with current crystallization stations.
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