ArticleCell death & disease2014
IRE1a constitutes a negative feedback loop with BMP2 and acts as a novel mediator in modulating osteogenic differentiation.
Article in Cell death & disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
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- Molecular mechanisms and targeted therapy of progranulin in metabolic diseases.Frontiers in endocrinology · 2025Review
- IRE1α pathway: A potential bone metabolism mediator.Cell proliferation · 2024Review
- Bone and the Unfolded Protein Response: In Sickness and in Health.Calcified tissue international · 2023Review
- The Role of Endoplasmic Reticulum Stress in Differentiation of Cells of Mesenchymal Origin.Biochemistry. Biokhimiia · 2022Review
- Supercritical Carbon Dioxide Decellularized Bone Matrix Seeded with Adipose-Derived Mesenchymal Stem Cells Accelerated Bone Regeneration.Biomedicines · 2021Article
- Extracellular vesicle-carried microRNA-27b derived from mesenchymal stem cells accelerates cutaneous wound healing via E3 ubiquitin ligase ITCH.Journal of cellular and molecular medicine · 2020Article
- Transmissible ER stress shapes the leukemic microenvironment.Oncotarget · 2019Article
- ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling.Cell communication and signaling : CCS · 2019Article
- Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells.Frontiers in chemistry · 2019Article
- ER stress signaling has an activating transcription factor 6α (ATF6)-dependent "off-switch".The Journal of biological chemistry · 2018Article
- Expression of Concern to IRE1a constitutes a negative feedback loop with BMP2 and acts as a novel mediator in modulating osteogenic differentiation.Cell death & disease · 2018Article
- An engineered, quantifiable in vitro model for analysing the effect of proteostasis-targeting drugs on tissue physical properties.Biomaterials · 2018Article
- Decreased MORF leads to prolonged endoplasmic reticulum stress in periodontitis-associated chronic inflammation.Cell death and differentiation · 2016Article
- New insights into the unfolded protein response in stem cells.Oncotarget · 2016Review
- Sphingosine Kinase 1 Protects Hepatocytes from Lipotoxicity via Down-regulation of IRE1α Protein Expression.The Journal of biological chemistry · 2015Article
- Transmission of ER stress response by ATF6 promotes endochondral bone growth.Journal of orthopaedic surgery and research · 2015Article
Corrections and comments
- Correction · 2015-04-16Error in Image · · see also: https://pubpeer.com/publications/3DF470C1215139B76F32F0A25AF2F8
- Expression of concern · 2018-11-09Concerns/Issues about Data · Concerns/Issues about Image · · see also: https://pubpeer.com/publications/3DF470C1215139B76F32F0A25AF2F8
- Erratum issued
- Expression of concern
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone morphogenetic protein 2 (BMP2) is known to activate unfolded protein response (UPR) signaling molecules, such as BiP (IgH chain-binding protein), PERK (PKR-like ER-resistant kinase), and IRE1α. Inositol-requiring enzyme-1a (IRE1a), as one of three unfolded protein sensors in UPR signaling pathways, can be activated during ER stress. Granulin-epithelin precursor (GEP) is an autocrine growth factor that has been implicated in embryonic development, tissue repair, tumorigenesis, and inflammation. However, the influence on IRE1a in BMP2-induced osteoblast differentiation has not yet been elucidated. Herein we demonstrate that overexpression of IRE1a inhibits osteoblast differentiation, as revealed by reduced activity of alkaline phosphatase (ALP) and osteocalcin; however, knockdown of IRE1a via the RNAi approach stimulates osteoblastogenesis. Mechanistic studies revealed that the expression of IRE1a during osteoblast was a consequence of JunB transcription factor binding to several AP1 sequence (TGAG/CTCA) in the 5'-flanking regulatory region of the IRE1a gene, followed by transcription. In addition, GEP induces IRE1a expressions and this induction of IRE1a by GEP depends on JunB. Furthermore, IRE1a inhibition of GEP-induced osteoblastogenesis relies on JunB. Besides, GEP is required for IRE1a inhibition of BMP2-induced bone formation. Collectively, these findings demonstrate that IRE1a negatively regulates BMP2-induced osteoblast differentiation and this IRE1a inhibition effect depends on GEP growth factor. Thus, IRE1a, BMP2, GEP growth factor, and JunB transcription factor form a regulatory loop and act in concert in the course of osteoblastogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.