ArticleInternational journal of molecular sciences2014
Detection of a specific biomarker for Epstein-Barr virus using a polymer-based genosensor.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Development of Epstein Barr virus biosensor by mimicking its infection mechanism for early detection of multiple sclerosis.Scientific reports · 2025Article
- Genosensors as an alternative diagnostic sensing approaches for specific detection of virus species: A review of common techniques and outcomes.Trends in analytical chemistry : TRAC · 2022Review
- Article
- Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma.Applied biochemistry and biotechnology · 2022Article
- Electrochemical preparation of monodisperse Pt nanoparticles on a grafted 4-aminothiophenol supporting layer for improving the MOR reaction.RSC advances · 2022Article
- Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus Detection.Journal of analytical methods in chemistry · 2022Article
- Portable Sensing Devices for Detection of COVID-19: A Review.IEEE sensors journal · 2021Article
- Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes.Biosensors · 2020Article
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Authors and funding
4 authors.
Funding
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Abstract
This paper describes methodology for direct and indirect detections of a specific oligonucleotide for Epstein-Barr virus (EBV) using electrochemical techniques. The sequence of oligonucleotide probe (EBV1) revealed a high sequence identity (100%) with the EBV genome. For the development of the genosensor, EBV1 was grafted to the platform sensitized with poly(4-aminothiophenol). After that, the hybridization reaction was carried out with the complementary target (EBV2) on the modified electrode surface using ethidium bromide as DNA intercalator. The oxidation peak currents of ethidium bromide increased linearly with the values of the concentration of the complementary sequences in the range from 3.78 to 756 µmol·L⁻¹. In nonstringent experimental conditions, this genosensor can detect 17.32 nmol·L⁻¹ (three independent experiments) of oligonucleotide target, discriminating between complementary and non-complementary oligonucleotides, as well as differentiating one-base mismatch, as required for detection of genetic diseases caused by point mutations. The biosensor also displayed high specificity to the EBV target with elimination of interference from mix (alanine, glucose, uric acid, ascorbic acid, bovine serum albumin (BSA), glutamate and glycine) and good stability (120 days). In addition, it was possible to observe differences between hybridized and non-hybridized surfaces through atomic force microscopy.
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