ArticleNature communications2014
Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration.
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Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
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Who cites it
32 citing papers in PubMed, 51 citations in OpenAlex.
- From Pits to Plaques: Electron Microscopy Reveals Clathrin Structural Plasticity in Trafficking, Sorting and Adhesion.Traffic (Copenhagen, Denmark) · 2026Review
- 1,2,4-Triazole-Acetamide Conjugates as hEGFR Inhibitors: Synthesis, Anticancer Evaluation, and In Silico Studies.Chemistry & biodiversity · 2026Article
- Clathrin Light Chain B Drives Hepatocellular Carcinoma Progression Through Dual Mechanisms: Small Extracellular Vesicle-Mediated Angiogenesis and the NF-κB-PCLAF Signaling Axis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- CLCa mediates a novel cross-talk between Wnt secretion and actin organization.Life science alliance · 2025Article
- Mechanistic divergences of endocytic clathrin-coated vesicle formation in mammals, yeasts and plants.Journal of cell science · 2024Review
- Tension-induced adhesion mode switching: the interplay between focal adhesions and clathrin-containing adhesion complexes.bioRxiv : the preprint server for biology · 2024Article
- Clathrin light chains CLCa and CLCb have non-redundant roles in epithelial lumen formation.Life science alliance · 2024Article
- IST1 regulates select recycling pathways.Traffic (Copenhagen, Denmark) · 2024Article
- Hallmark guided identification and characterization of a novel immune-relevant signature for prognostication of recurrence in stage I-III lung adenocarcinoma.Genes & diseases · 2023Article
- Pancreatic Ductal Cell-Derived Extracellular Vesicles Are Effective Drug Carriers to Enhance Paclitaxel's Efficacy in Pancreatic Cancer Cells through Clathrin-Mediated Endocytosis.International journal of molecular sciences · 2022Article
- MAG2 and MAL Regulate Vesicle Trafficking and Auxin Homeostasis With Functional Redundancy.Frontiers in plant science · 2022Article
- Identification of Genes Related to Cold Tolerance and Novel Genetic Markers for Molecular Breeding in Taiwan Tilapia (Animals : an open access journal from MDPI · 2021Article
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- Clathrin Light Chains: Not to Be Taken so Lightly.Frontiers in cell and developmental biology · 2021Review
- Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Application of advances in endocytosis and membrane trafficking to drug delivery.Advanced drug delivery reviews · 2020Review
- Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension.Nature communications · 2019Article
- Taming the Triskelion: Bacterial Manipulation of Clathrin.Microbiology and molecular biology reviews : MMBR · 2019Review
- A unique role for clathrin light chain A in cell spreading and migration.Journal of cell science · 2019Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Sophia R Majeed1] Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California 94143, USA [2] Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143, USA [3] Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA [4] The G.W. Hooper Foundation, University of California San Francisco, San Francisco, California 94143, USA.
Lavanya Vasudevan1] Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California 94143, USA [2] Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143, USA [3] Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA [4] The G.W. Hooper Foundation, University of California San Francisco, San Francisco, California 94143, USA [5].
Chih-Ying Chen1] Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California 94143, USA [2] Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143, USA [3] Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA [4] The G.W. Hooper Foundation, University of California San Francisco, San Francisco, California 94143, USA [5].
Yi Luo1] Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA [2].
Jorge A Torres1] Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California 94143, USA [2] Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143, USA [3] Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA [4] The G.W. Hooper Foundation, University of California San Francisco, San Francisco, California 94143, USA.
Timothy M Evans1] Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California 94143, USA [2] Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143, USA [3] Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA [4] The G.W. Hooper Foundation, University of California San Francisco, San Francisco, California 94143, USA.
Andrew SharkeyDepartment of Pathology and Centre for Trophoblast Research, University of Cambridge, Cambridge CB2 1QP, UK.
Amy B Foraker1] Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California 94143, USA [2] Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143, USA [3] Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA [4] The G.W. Hooper Foundation, University of California San Francisco, San Francisco, California 94143, USA.
Nicole M L Wong1] Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California 94143, USA [2] Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143, USA [3] Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA [4] The G.W. Hooper Foundation, University of California San Francisco, San Francisco, California 94143, USA.
Christopher Esk1] Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California 94143, USA [2] Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143, USA [3] Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA [4] The G.W. Hooper Foundation, University of California San Francisco, San Francisco, California 94143, USA.
Theresa A FreemanDepartment of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Ashley MoffettDepartment of Pathology and Centre for Trophoblast Research, University of Cambridge, Cambridge CB2 1QP, UK.
James H KeenDepartment of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Frances M Brodsky1] Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California 94143, USA [2] Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143, USA [3] Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA [4] The G.W. Hooper Foundation, University of California San Francisco, San Francisco, California 94143, USA.
University of California, San Francisco · USThomas Jefferson University · USUniversity of Cambridge · GB
Funding
X-Ray Crystallography and Macromolecular CharacterizationP30CA056036 · NCI · THOMAS JEFFERSON UNIVERSITY · PI DANIEL P SILVER · 1995 to 2026
$94.8MPHARMACEUTICAL SCIENCES AND PHARMACOGENOMICST32GM007175 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI AHITUV, NADAV, KROETZ, DEANNA L · 1985 to 2021
$7.1MTHE MOLECULAR MECHANISM OF CLATHRIN ASSEMBLYR01GM038093 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BRODSKY, FRANCES M. · 1988 to 2014
$5.0MRegulation of Clathrin and Adaptor FunctionR01GM049217 · NIGMS · THOMAS JEFFERSON UNIVERSITY · PI KEEN, JAMES H · 1994 to 2016
$3.8MThe role of clathrin and the Hip proteins in breast cancer metastasisF31CA171594 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MAJEED, SOPHIA · 2012 to 2014
$112kNCI NIH HHS F31 CA171594NCI NIH HHS NCI F31 CA171594NCI NIH HHS P30 CA056036NIGMS NIH HHS GM038093NIGMS NIH HHS GM049217NIGMS NIH HHS R01 GM038093NIGMS NIH HHS R01 GM049217NIGMS NIH HHS T32 GM007175NIGMS NIH HHS T32 GM07175
Abstract
The clathrin light chain (CLC) subunits participate in several membrane traffic pathways involving both clathrin and actin, through binding the actin-organizing huntingtin-interacting proteins (Hip). However, CLCs are dispensable for clathrin-mediated endocytosis of many cargoes. Here we observe that CLC depletion affects cell migration through Hip binding and reduces surface expression of β1-integrin by interference with recycling following normal endocytosis of inactive β1-integrin. CLC depletion and expression of a modified CLC also inhibit the appearance of gyrating (G)-clathrin structures, known mediators of rapid recycling of transferrin receptor from endosomes. Expression of the modified CLC reduces β1-integrin and transferrin receptor recycling, as well as cell migration, implicating G-clathrin in these processes. Supporting a physiological role for CLC in migration, the CLCb isoform of CLC is upregulated in migratory human trophoblast cells during uterine invasion. Together, these studies establish CLCs as mediating clathrin-actin interactions needed for recycling by G-clathrin during migration.
Indexed as
Cell MovementEndocytosisActinsAnimalsCattleClathrin Heavy ChainsClathrin Light ChainsFemaleFocal AdhesionsGene Expression RegulationGene Knockdown TechniquesHeLa CellsHumansIntegrin beta1Models, BiologicalOligonucleotide Array Sequence AnalysisActinsClathrin Heavy ChainsClathrin Light ChainsIntegrin beta1RNA, MessengerRNA, Small Interfering
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