ArticleJournal of lipid research2014
Spatiotemporal lipid profiling during early embryo development of Xenopus laevis using dynamic ToF-SIMS imaging.
Article in Journal of lipid research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Lipid Changes in the Peri-Implantation Period with Mass Spectrometry Imaging: A Systematic Review.Life (Basel, Switzerland) · 2023Review
- Comparative Metabolomics of Small Molecules Specifically Expressed in the Dorsal or Ventral Marginal Zones in Vertebrate Gastrula.Metabolites · 2022Article
- Mass Spectrometry Imaging Shows Modafinil, A Student Study Drug, Changes the Lipid Composition of the Fly Brain.Angewandte Chemie (International ed. in English) · 2021Article
- Interplay between Cocaine, Drug Removal, and Methylphenidate Reversal on Phospholipid Alterations inACS chemical neuroscience · 2020Article
- Lipid Alterations during Zebrafish Embryogenesis Revealed by Dynamic Mass Spectrometry Profiling with C=C Specificity.Journal of the American Society for Mass Spectrometry · 2019Article
- Imaging with mass spectrometry, the next frontier in sphingolipid research? A discussion on where we stand and the possibilities ahead.Chemistry and physics of lipids · 2019Review
- Observation of endoplasmic reticulum tubules via TOF-SIMS tandem mass spectrometry imaging of transfected cells.Biointerphases · 2018Article
- In Situ Microprobe Single-Cell Capillary Electrophoresis Mass Spectrometry: Metabolic Reorganization in Single Differentiating Cells in the Live Vertebrate (Xenopus laevis) Embryo.Analytical chemistry · 2017Article
- New-generation mass spectrometry expands the toolbox of cell and developmental biology.Genesis (New York, N.Y. : 2000) · 2017Review
- Metabolomic approach for identifying and visualizing molecular tissue markers in tadpoles of Xenopus tropicalis by mass spectrometry imaging.Biology open · 2016Article
- Article
- Review
- Mass spectrometric imaging of brain tissue by time-of-flight secondary ion mass spectrometry--How do polyatomic primary beams C₆₀⁺, Ar₂₀₀₀⁺, water-doped Ar₂₀₀₀⁺ and (H₂O)₆₀₀₀⁺ compare?Rapid communications in mass spectrometry : RCM · 2015Article
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7 authors.
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Abstract
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging has been used for the direct analysis of single intact Xenopus laevis embryo surfaces, locating multiple lipids during fertilization and the early embryo development stages with subcellular lateral resolution (∼4 μm). The method avoids the complicated sample preparation for lipid analysis of the embryos, which requires selective chemical extraction of a pool of samples and chromatographic separation, while preserving the spatial distribution of biological species. The results show ToF-SIMS is capable of profiling multiple components (e.g., glycerophosphocholine, SM, cholesterol, vitamin E, diacylglycerol, and triacylglycerol) in a single X. laevis embryo. We observe lipid remodeling during fertilization and early embryo development via time course sampling. The study also reveals the lipid distribution on the gamete fusion site. The methodology used in the study opens the possibility of studying developmental biology using high resolution imaging MS and of understanding the functional role of the biological molecules.
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