ArticleJournal of inflammation (London, England)2014
Chorioamnionitis (ChA) modifies CX3CL1 (fractalkine) production by human amniotic epithelial cells (HAEC) under normoxic and hypoxic conditions.
Article in Journal of inflammation (London, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Alterations in CX3CL1 Levels and Its Role in Viral Pathogenesis.International journal of molecular sciences · 2024Review
- Role of chemokines in early pregnancy loss.Experimental and therapeutic medicine · 2022Article
- MicroRNA-424 regulates the expression of CX3CL1 (fractalkine) in human microvascular endothelial cells duringBiochemistry and biophysics reports · 2021Article
- Is there an inflammatory stimulus to human term labour?PloS one · 2021Article
- Data-driven discovery of mid-pregnancy immune markers associated with maternal lifetime stress: results from an urban pre-birth cohort.Stress (Amsterdam, Netherlands) · 2020Article
- Anti-inflammatory effects of dexmedetomidine on human amnion-derived WISH cells.International journal of medical sciences · 2020Article
- Placental CX3CL1 is Deregulated by Angiotensin II and Contributes to a Pro-Inflammatory Trophoblast-Monocyte Interaction.International journal of molecular sciences · 2019Article
- Human Amnion Epithelial Cell Therapy for Chronic Liver Disease.Stem cells international · 2019Review
- The role of CX3CL1 in fetal-maternal interaction during human gestation.Cell adhesion & migration · 2016Review
- Placental fractalkine mediates adhesion of THP-1 monocytes to villous trophoblast.Histochemistry and cell biology · 2015Article
- Aspirin Action in Endothelial Cells: Different Patterns of Response Between Chemokine CX3CL1/CX3CR1 and TNF-α/TNFR1 Signaling Pathways.Cardiovascular drugs and therapy · 2015Article
- Placental fractalkine is up-regulated in severe early-onset preeclampsia.The American journal of pathology · 2015Article
- Cellular Regulation of the Uterine Microenvironment That Enables Embryo Implantation.Frontiers in immunology · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChemokine CX3CL1 possesses unique properties, including combined adhesive and chemotactic functions. Human amniotic epithelial cells (HAEC) show expression of CX3CL1 receptor (CX3CR1) and produce CX3CL1 in response to both physiologic and pathologic stimuli. Chorioamnionitis (ChA) is a common complication of pregnancy and labour. ChA is often accompanied by local hypoxia because of the high oxygen consumption at the site of inflammation. We examined comparatively (ChA-complicated vs. normal pregnancy) CX3CR1 expression and the effects of hypoxia, lipopolysaccharide (LPS), and CX3CR1 blockade on CX3CL1 production in HAEC cultured in vitro.
methodsHAEC have been isolated using trypsinization, and cultured under normoxia (20% O2) vs. hypoxia (5% O2). According to the experimental design, LPS (1 μg/ml) and neutralizing anti-CX3CR1 antibodies were added at respective time points. Mean CX3CL1 concentration in the supernatant samples were determined by ELISA. Expression of immunostained CX3CR1 was analyzed using quantitative morphometry.
resultsWe have found that the mean levels of CX3CL1 and CX3CR1 expression were remarkably (p < 0.05) higher in ChA, compared to normal pregnancy. Significantly increased expression of CX3CR1 was observed in ChA during both normoxia and hypoxia. Hypoxia exposure produced decrease in the mean concentration of CX3CL1 in both groups, however this reduction was stronger in normal pregnancy. In normoxia, LPS-evoked rise in the mean concentration of CX3CL1 was higher (p < 0.05) in normal pregnancy. This response was positively correlated with CX3CR1 expression. Blockade of CX3CR1 canceled the secretory response to LPS in all groups.
conclusionsChA-complicated pregnancy up-regulates CX3CR1 in HAEC cultured in vitro with simultaneous increase in CX3CL1 production. Hypoxia-resistant production of CX3CL1 may be responsible for ChA-related complications of pregnancy and labor.
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