ArticleThe Korean journal of parasitology2014
Down-regulation of cellulose synthase inhibits the formation of endocysts in Acanthamoeba.
Article in The Korean journal of parasitology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- Molecular evolution and adaptations ofFrontiers in cellular and infection microbiology · 2026Review
- Encystment and Excystment Processes inPathogens (Basel, Switzerland) · 2025Review
- Genetic manipulation of giant viruses and their host, Acanthamoeba castellanii.Nature protocols · 2024Review
- Evolution of giant pandoravirus revealed by CRISPR/Cas9.Nature communications · 2023Article
- Biological characteristics and pathogenicity ofFrontiers in microbiology · 2023Review
- Opportunistic free-living amoebal pathogens.Pathogens and global health · 2022Article
- Anti-PeerJ · 2022Article
- RNA Sequencing of Medusavirus Suggests Remodeling of the Host Nuclear Environment at an Early Infection Stage.Microbiology spectrum · 2021Article
- Article
- The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba.Parasites & vectors · 2020Article
- Encystment Induces Down-Regulation of an Acetyltransferase-Like Gene inPathogens (Basel, Switzerland) · 2020Article
- Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella Effector.Cell host & microbe · 2020Article
- The most abundant cyst wall proteins of Acanthamoeba castellanii are lectins that bind cellulose and localize to distinct structures in developing and mature cyst walls.PLoS neglected tropical diseases · 2019Article
- Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture.Scientific reports · 2019Article
- Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii.Parasites & vectors · 2018Article
- DNA Methylation of Gene Expression inThe Korean journal of parasitology · 2017Article
- Cellulose degradation: a therapeutic strategy in the improved treatment of Acanthamoeba infections.Parasites & vectors · 2015Review
- An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment.Parasite (Paris, France) · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acanthamoeba cysts are resistant to unfavorable physiological conditions and various disinfectants. Acanthamoeba cysts have 2 walls containing various sugar moieties, and in particular, one third of the inner wall is composed of cellulose. In this study, it has been shown that down-regulation of cellulose synthase by small interfering RNA (siRNA) significantly inhibits the formation of mature Acanthamoeba castellanii cysts. Calcofluor white staining and transmission electron microscopy revealed that siRNA transfected amoeba failed to form an inner wall during encystation and thus are likely to be more vulnerable. In addition, the expression of xylose isomerase, which is involved in cyst wall formation, was not altered in cellulose synthase down-regulated amoeba, indicating that cellulose synthase is a crucial factor for inner wall formation by Acanthamoeba during encystation.
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