ArticleProceedings of the National Academy of Sciences of the United States of America2014
Combining linkage and association mapping identifies RECEPTOR-LIKE PROTEIN KINASE1 as an essential Arabidopsis shoot regeneration gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed.
- Stem cell activity is linked to a transient acquisition of pluripotency during callus proliferation.Nature communications · 2026Article
- Strain, procedures, and tools for reproducible genetic transformation and genome editing of the emerging plant model Spirodela polyrhiza.The New phytologist · 2026Article
- Rational design of induced regeneration via somatic embryogenesis in the absence of exogenous phytohormones.The Plant cell · 2025Article
- A Comprehensive Analysis on the Regulatory Network Underlying Callus Induction and Adventitious Organogenesis Process in Stem ofInternational journal of molecular sciences · 2025Article
- Developmental regulatory genes in recalcitrant forest trees: advances in somatic regeneration and genetic transformation.Frontiers in plant science · 2025Review
- Association mapping and identification of candidate genes for callus induction and regeneration using sorghum mature seeds.Frontiers in plant science · 2025Article
- Genomic basis determining root system architecture in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Autonomous differentiation of transgenic cells requiring no external hormone application: the endogenous gene expression and phytohormone behaviors.Frontiers in plant science · 2024Article
- QTLs Related to Rice Callus Regeneration Ability: Localization and Effect Verification ofCells · 2022Article
- Genome-Wide Identification and Characterization ofFrontiers in genetics · 2022Article
- Current status and trends in forest genomics.Forestry research · 2022Review
- Histidine kinase inhibitors impair shoot regeneration inFrontiers in plant science · 2022Article
- Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs.International journal of molecular sciences · 2021Review
- Highly efficient and genotype-independent barley gene editing based on anther culture.Plant communications · 2021Article
- Variability in somatic embryo-forming capacity of spinach.Scientific reports · 2020Article
- The genetic framework of shoot regeneration in Arabidopsis comprises master regulators and conditional fine-tuning factors.Communications biology · 2020Article
- Natural Variation in Plant Pluripotency and Regeneration.Plants (Basel, Switzerland) · 2020Review
- An Artificial Conversion of Roots into Organs with Shoot Stem Characteristics by Inducing Two Transcription Factors.iScience · 2020Article
- Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration.Plants (Basel, Switzerland) · 2020Review
- Linkage mapping and genome-wide association study reveals conservative QTL and candidate genes for Fusarium rot resistance in maize.BMC genomics · 2020Article
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9 authors.
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Abstract
De novo shoot organogenesis (i.e., the regeneration of shoots on nonmeristematic tissue) is widely applied in plant biotechnology. However, the capacity to regenerate shoots varies highly among plant species and cultivars, and the factors underlying it are still poorly understood. Here, we evaluated the shoot regeneration capacity of 88 Arabidopsis thaliana accessions and found that the process is blocked at different stages in different accessions. We show that the variation in regeneration capacity between the Arabidopsis accessions Nok-3 and Ga-0 is determined by five quantitative trait loci (QTL): REG-1 to REG-5. Fine mapping by local association analysis identified RECEPTOR-LIKE PROTEIN KINASE1 (RPK1), an abscisic acid-related receptor, as the most likely gene underlying REG-1, which was confirmed by quantitative failure of an RPK1 mutation to complement the high and low REG-1 QTL alleles. The importance of RPK1 in regeneration was further corroborated by mutant and expression analysis. Altogether, our results show that association mapping combined with linkage mapping is a powerful method to discover important genes implicated in a biological process as complex as shoot regeneration.
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