ArticleJournal of clinical microbiology2014
Rapid detection and identification of mucormycetes in bronchoalveolar lavage samples from immunocompromised patients with pulmonary infiltrates by use of high-resolution melt analysis.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- Sensitivity and Specificity of Polymerase Chain Reaction in Blood and Bronchoalveolar Lavage Samples for Mucormycosis: A Bayesian Diagnostic Test Accuracy Meta-Analysis.Mycopathologia · 2025Pooled it
- A Rare Case ofInfectious disease reports · 2025Article
- Development and evaluation of a Pan-Mucorales Real-time PCR and a multiplex Real-time PCR for detection and identification ofJournal of clinical microbiology · 2025Article
- The diagnosis of mucormycosis by PCR in patients at risk: a systematic review and meta-analysis.EClinicalMedicine · 2025Article
- Interlaboratory assays from the fungal PCR Initiative and the Modimucor Study Group to improve qPCR detection of Mucorales DNA in serum: one more step toward standardization.Journal of clinical microbiology · 2025Article
- Recent Advances in Diagnostic Approaches for Mucormycosis.Journal of fungi (Basel, Switzerland) · 2024Review
- Molecular Diagnostics for Invasive Fungal Diseases: Current and Future Approaches.Journal of fungi (Basel, Switzerland) · 2024Review
- Nucleic-Acid-Based Molecular Fungal Diagnostics: A Way to a Better Future.Diagnostics (Basel, Switzerland) · 2024Review
- The SSS revolution in fungal diagnostics: speed, simplicity and sensitivity.British medical bulletin · 2023Article
- Comparative genomics predict specific genes in potential mucorales identification.Archives of microbiology · 2023Article
- Epidemiology, Modern Diagnostics, and the Management of Mucorales Infections.Journal of fungi (Basel, Switzerland) · 2023Review
- Clinical Features and Treatment Progress of Invasive Mucormycosis in Patients with Hematological Malignancies.Journal of fungi (Basel, Switzerland) · 2023Review
- Rapid detection of Mucorales based on recombinase polymerase amplification and real-time PCR.Frontiers in microbiology · 2023Article
- Detection and identification ofFrontiers in cellular and infection microbiology · 2023Article
- A Rapid and Specific Real-Time PCR Assay for the Detection of Clinically Relevant Mucorales Species.International journal of molecular sciences · 2022Article
- Evaluation of the Diagnostic Accuracy and Clinical Utility of Fungal Profile Plus Polymerase Chain Reaction Assay in Pulmonary Infections.Open forum infectious diseases · 2022Article
- Machine learning to improve the interpretation of intercalating dye-based quantitative PCR results.Scientific reports · 2022Article
- The impact of SARS-CoV-2 on the sudden onset of Mucormycosis in the Indian subcontinent-A review.Journal of family medicine and primary care · 2022Review
- Analytical Performance of the Commercial MucorGeniusJournal of fungi (Basel, Switzerland) · 2022Article
- Mucormycosis: A comparative update between conventional and molecular diagnosis strategies.Current medical mycology · 2022Review
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid differential diagnostics of pulmonary infiltrates suspected of invasive fungal disease in an immunocompromised host and early initiation of effective antifungal therapy are crucial for patient outcomes. There are no serological tests available to detect mucormycetes; therefore, PCR-based methods are highly suitable. We validated our previously published PCR followed by high-resolution melt analysis (PCR/HRMA) to detect Rhizopus spp., Rhizomucor pusillus, Lichtheimia corymbifera, and Mucor spp. in bronchoalveolar lavage (BAL) samples from immunocompromised patients who were at risk of invasive fungal disease. All PCR/HRMA-positive samples were retested using novel real-time quantitative PCR (RQ PCR) assays specific to the species identified. In total, between January 2009 and December 2012 we analyzed 99 BAL samples from 86 patients with pulmonary abnormalities using PCR/HRMA. Ninety (91%) BAL samples were negative, and 9 (9%) samples were positive. The sensitivity and specificity of PCR/HRMA were 100% and 93%, respectively. By combining the positive results of PCR/HRMA with positive RQ PCR results, the specificity was raised to 98%. PCR/HRMA, due to its high negative predictive value (99%), represents a fast and reliable tool for routine BAL sample screening for the differential diagnosis of pulmonary infiltrates in immunocompromised patients for the four most clinically important mucormycetes.
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