ArticleJournal of clinical microbiology2014
Genomic variation between Campylobacter jejuni isolates associated with milk-borne-disease outbreaks.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Genomic insights into zoonotic transmission and antimicrobial resistance in Campylobacter jejuni from farm to fork: a one health perspective.Gut pathogens · 2022Review
- Molecular Epidemiological Evidence Implicates Cattle as a Primary Reservoir ofPathogens (Basel, Switzerland) · 2022Article
- Genomic Analysis Reveals That Isolation Temperature on Selective Media Introduces Genetic Variation inPathogens (Basel, Switzerland) · 2022Article
- Impact of Ceftiofur Administration in Steers on the Prevalence and Antimicrobial Resistance ofMicroorganisms · 2021Article
- Comparison of Molecular Subtyping and Antimicrobial Resistance Detection Methods Used in a Large Multistate Outbreak of Extensively Drug-Resistant Campylobacter jejuni Infections Linked to Pet Store Puppies.Journal of clinical microbiology · 2020Article
- Whole-Genome Sequencing to Detect Numerous Campylobacter jejuni Outbreaks and Match Patient Isolates to Sources, Denmark, 2015-2017.Emerging infectious diseases · 2020Article
- Whole-Genome Sequencing and Bioinformatic Analysis of Environmental, Agricultural, and HumanFrontiers in microbiology · 2020Article
- Whole genome sequencing and metagenomics for outbreak investigation, source attribution and risk assessment of food-borne microorganisms.EFSA journal. European Food Safety Authority · 2019Article
- Epidemiological Typing of Serratia marcescens Isolates by Whole-Genome Multilocus Sequence Typing.Journal of clinical microbiology · 2019Article
- Population Genetics and Characterization of Campylobacter jejuni Isolates from Western Jackdaws and Game Birds in Finland.Applied and environmental microbiology · 2019Article
- Genomic epidemiology of clinical Campylobacter spp. at a single health trust site.Microbial genomics · 2018Article
- Whole-Genome Sequencing in Epidemiology of Campylobacter jejuni Infections.Journal of clinical microbiology · 2017Review
- Genomic insights from whole genome sequencing of four clonal outbreak Campylobacter jejuni assessed within the global C. jejuni population.BMC genomics · 2016Article
- Molecular Assay Validation Using Genomic Sequence Databases.Journal of clinical microbiology · 2016Article
- Next-Generation Epidemiology: Using Real-Time Core Genome Multilocus Sequence Typing To Support Infection Control Policy.Journal of clinical microbiology · 2016Article
- A large outbreak of Campylobacter jejuni infection in a university college caused by chicken liver pâté, Australia, 2013.Epidemiology and infection · 2016Article
- Monomorphic genotypes within a generalist lineage ofMicrobial genomics · 2016Article
- Partial Failure of Milk Pasteurization as a Risk for the Transmission of Campylobacter From Cattle to Humans.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015Article
- Milk Modulates Campylobacter Invasion into Caco-2 Intestinal Epithelial Cells.European journal of microbiology & immunology · 2015Article
- Global Epidemiology of Campylobacter Infection.Clinical microbiology reviews · 2015Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial genome sequencing has led to the development of new approaches for the analysis of food-borne epidemics and the exploration of the relatedness of outbreak-associated isolates and their separation from nonassociated isolates. Using Illumina technology, we sequenced a total of six isolates (two from patients, two from raw bulk milk, and two from dairy cattle) associated with a milk-borne Campylobacter jejuni outbreak in a farming family and compared their genomes. These isolates had identical pulsed-field gel electrophoresis (PFGE) types, and their multilocus sequence typing (MLST) type was ST-50. We used the Ma_1 isolate (milk) as the reference, and its genome was assembled and tentatively ordered using the C. jejuni NCTC 11168 genome as the scaffold. Using whole-genome MLST (wgMLST), we identified a total of three single-nucleotide polymorphisms (SNPs) and differences in poly(G or C) or poly(A or T) tracts in 12 loci among the isolates. Several new alleles not present in the database were detected. In contrast, the sequences of the unassociated C. jejuni strains P14 and 1-12S (both ST-50) differed by 420 to 454 alleles from the epidemic-associated isolates. We found that the fecal contamination of bulk tank milk occurred by highly related sequence variants of C. jejuni, which are reflected as SNPs and differences in the length of the poly(A or T) tracts. Poly(G or C) tracts are reversibly variable and are thus unstable markers for comparison. Further, unrelated strains of ST-50 were clearly separated from the outbreak-associated isolates, indicating that wgMLST is an excellent tool for analysis. In addition, other useful data related to the genes and genetic systems of the isolates were obtained.
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