ArticleJournal of clinical microbiology2014
Use of the Bruker MALDI Biotyper for identification of molds in the clinical mycology laboratory.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Evaluation of multiple extraction and inactivation methods for the rapid identification of filamentous fungi using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Microbiology spectrum · 2026Article
- Speciation analysis of fungi by liquid atmospheric pressure MALDI mass spectrometry.Analytical and bioanalytical chemistry · 2025Article
- Comparison of the BluePoint MoldID oligonucleotide array and Bruker Biotyper MALDI-TOF MS for the identification of filamentous fungi.Journal of clinical microbiology · 2025Article
- Identification of Challenging Dermatophyte Species Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.Journal of fungi (Basel, Switzerland) · 2025Article
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: A breakthrough in rapid and precise mold identification.Current medical mycology · 2025Article
- Scedosporiosis and lomentosporiosis: modern perspectives on these difficult-to-treat rare mold infections.Clinical microbiology reviews · 2024Review
- An easy adjustment of instrument settings ('Peak MALDI') improves identification of organisms by MALDI-ToF mass spectrometry.Scientific reports · 2023Article
- Bioprotective potential of lactic acid bacteria for Salmonella biocontrol in vitro.Veterinary research communications · 2023Article
- Mass spectrometry-based proteomics as an emerging tool in clinical laboratories.Clinical proteomics · 2023Review
- Review
- Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification ofFrontiers in microbiology · 2022Article
- Performance of a Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Testing Algorithm for the Rapid Identification of Clinical Filamentous Molds.Frontiers in cellular and infection microbiology · 2022Article
- Identification of Molds with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Performance of the Newly Developed MSI-2 Application in Comparison with the Bruker Filamentous Fungi Database and MSI-1.Journal of clinical microbiology · 2021Article
- Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Ocular Infections.Clinical microbiology reviews · 2021Review
- MALDI-TOF MS in a Medical Mycology Laboratory: On Stage and Backstage.Microorganisms · 2021Review
- Matrix-Assisted Laser Desorption Ionization Time-of-Flight for Fungal Identification.Clinics in laboratory medicine · 2021Review
- Rapid identification of fungi from respiratory samples by Bruker Biotyper matrix-assisted laser desorption/ionisation time-of-flight using ID-FUNGI plates.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Article
- Developing Two Rapid Protein Extraction Methods Using Focused-Ultrasonication and Zirconia-Silica Beads for Filamentous Fungi Identification by MALDI-TOF MS.Frontiers in cellular and infection microbiology · 2021Article
- Epidemiology and Diagnostic Perspectives of Dermatophytoses.Journal of fungi (Basel, Switzerland) · 2020Review
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7 authors.
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Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is increasingly used for the identification of bacteria and fungi in the diagnostic laboratory. We evaluated the mold database of Bruker Daltonik (Bremen, Germany), the Filamentous Fungi Library 1.0. First, we studied 83 phenotypically and molecularly well-characterized, nondermatophyte, nondematiaceous molds from a clinical strain collection. Using the manufacturer-recommended interpretation criteria, genus and species identification rates were 78.3% and 54.2%, respectively. Reducing the species cutoff from 2.0 to 1.7 significantly increased species identification to 71.1% without increasing misidentifications. In a subsequent prospective study, 200 consecutive clinical mold isolates were identified by the MALDI Biotyper and our conventional identification algorithm. Discrepancies were resolved by ribosomal DNA (rDNA) internal transcribed spacer region sequence analysis. For the MALDI Biotyper, genus and species identification rates were 83.5% and 79.0%, respectively, when using a species cutoff of 1.7. Not identified were 16.5% of the isolates. Concordant genus and species assignments of MALDI-TOF MS and the conventional identification algorithm were observed for 98.2% and 64.2% of the isolates, respectively. Four erroneous species assignments were observed using the MALDI Biotyper. The MALDI Biotyper seems highly reliable for the identification of molds when using the Filamentous Fungi Library 1.0 and a species cutoff of 1.7. However, expansion of the database is required to reduce the number of nonidentified isolates.
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