ArticleLab on a chip2014
Highly permeable silicon membranes for shear free chemotaxis and rapid cell labeling.
Article in Lab on a chip, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- Shear Conditioning Promotes Microvascular Endothelial Barrier Resilience in a Human BBB-on-a-Chip Model of Systemic Inflammation Leading to Astrogliosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A tissue chip with integrated digital immunosensors: In situ brain endothelial barrier cytokine secretion monitoring.Biosensors & bioelectronics · 2023Article
- A photonic biosensor-integrated tissue chip platform for real-time sensing of lung epithelial inflammatory markers.Lab on a chip · 2023Article
- The Modular µSiM: A Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro.Advanced healthcare materials · 2022Article
- Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform.Communications biology · 2022Article
- Article
- Endothelial cell apicobasal polarity coordinates distinct responses to luminally versus abluminally delivered TNF-α in a microvascular mimetic.Integrative biology : quantitative biosciences from nano to macro · 2020Article
- Microfluidic devices for neutrophil chemotaxis studies.Journal of translational medicine · 2020Review
- Chemotactic Responses of Jurkat Cells in Microfluidic Flow-Free Gradient Chambers.Micromachines · 2020Article
- Microvascular Mimetics for the Study of Leukocyte-Endothelial Interactions.Cellular and molecular bioengineering · 2020Article
- Ultrathin Silicon Membranes forACS nano · 2020Article
- Tangential flow microfluidics for the capture and release of nanoparticles and extracellular vesicles on conventional and ultrathin membranes.Advanced materials technologies · 2019Article
- An in vitro platform for elucidating the molecular genetics of S. aureus invasion of the osteocyte lacuno-canalicular network during chronic osteomyelitis.Nanomedicine : nanotechnology, biology, and medicine · 2019Article
- A silicon nanomembrane platform for the visualization of immune cell trafficking across the human blood-brain barrier under flow.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Article
- Ultrathin Dual-Scale Nano- and Microporous Membranes for Vascular Transmigration Models.Small (Weinheim an der Bergstrasse, Germany) · 2019Article
- Protein Separation and Hemocompatibility of Nitride Membranes in Microfluidic Filtration Systems.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2018Article
- Use of porous membranes in tissue barrier and co-culture models.Lab on a chip · 2018Review
- TEM Tomography of Pores with Application to Computational Nanoscale Flows in Nanoporous Silicon Nitride (NPN).Membranes · 2018Article
- A high-performance polydimethylsiloxane electrospun membrane for cell culture in lab-on-a-chip.Biomicrofluidics · 2018Article
- Porous Substrates Promote Endothelial Migration at the Expense of Fibronectin Fibrillogenesis.ACS biomaterials science & engineering · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Microfluidic systems are powerful tools for cell biology studies because they enable the precise addition and removal of solutes in small volumes. However, the fluid forces inherent in the use of microfluidics for cell cultures are sometimes undesirable. An important example is chemotaxis systems where fluid flow creates well-defined and steady chemotactic gradients but also pushes cells downstream. Here we demonstrate a chemotaxis system in which two chambers are separated by a molecularly thin (15 nm), transparent, and nanoporous silicon membrane. One chamber is a microfluidic channel that carries a flow-generated gradient while the other chamber is a shear-free environment for cell observation. The molecularly thin membranes provide effectively no resistance to molecular diffusion between the two chambers, making them ideal elements for creating flow-free chambers in microfluidic systems. Analytical and computational flow models that account for membrane and chamber geometry, predict shear reduction of more than five orders of magnitude. This prediction is confirmed by observing the pure diffusion of nanoparticles in the cell-hosting chamber despite high input flow (Q = 10 μL min(-1); vavg ~ 45 mm min(-1)) in the flow chamber only 15 nm away. Using total internal reflection fluorescence (TIRF) microscopy, we show that a flow-generated molecular gradient will pass through the membrane into the quiescent cell chamber. Finally we demonstrate that our device allows us to expose migrating neutrophils to a chemotactic gradient or fluorescent label without any influence from flow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.