ArticleBiochemistry2014
Characterizing the dynamics of the leader-linker interaction in the glycine riboswitch with site-directed spin labeling.
Article in Biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Paramagnetic Chemical Probes for Studying Biological Macromolecules.Chemical reviews · 2022Review
- Development and characterization of a glycine biosensor system for fine-tuned metabolic regulation in Escherichia coli.Microbial cell factories · 2022Article
- Lanthanide Tagging of Oligonucleotides to Nucleobase for Paramagnetic NMR.Chembiochem : a European journal of chemical biology · 2020Article
- A metabolically engineered spin-labeling approach for studying glycans on cells.Chemical science · 2020Article
- Do the P1 and P2 hairpins of the Guanidine-II riboswitch interact?Nucleic acids research · 2020Article
- Site-Directed Spin Labeling of RNA with aMolecules (Basel, Switzerland) · 2019Article
- A Glycine Riboswitch inFrontiers in microbiology · 2018Article
- Article
- Singlet glycine riboswitches bind ligand as well as tandem riboswitches.RNA (New York, N.Y.) · 2016Article
- Complementary-addressed site-directed spin labeling of long natural RNAs.Nucleic acids research · 2016Article
- Fluorescence-Based Strategies to Investigate the Structure and Dynamics of Aptamer-Ligand Complexes.Frontiers in chemistry · 2016Review
- Consistent global structures of complex RNA states through multidimensional chemical mapping.eLife · 2015Article
- Ligand binding by the tandem glycine riboswitch depends on aptamer dimerization but not double ligand occupancy.RNA (New York, N.Y.) · 2014Article
- Continuous wave W- and D-band EPR spectroscopy offer "sweet-spots" for characterizing conformational changes and dynamics in intrinsically disordered proteins.Biochemical and biophysical research communications · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Site-directed spin labeling with continuous wave electron paramagnetic resonance (EPR) spectroscopy was utilized to characterize dynamic features of the kink-turn motif formed through a leader-linker interaction in the Vibrio cholerae glycine riboswitch. Efficient incorporation of spin-labels into select sites within the phosphate backbone of the leader-linker region proceeded via splinted ligation of chemically synthesized spin-labeled oligonucleotides to in vitro transcribed larger RNA fragments. The resultant nitroxide EPR line shapes have spectral characteristics consistent with a kink-turn motif and reveal differential backbone dynamics that are modulated by the presence of magnesium, potassium, and glycine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.