Evidence map›Paper›PMID 24849765›Full record

ArticleJournal of clinical laboratory analysis2015

High-Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene.

Enzhu Jiang, Fengrui Li, Chenchen Jing, Pei Li, Honggang Cui, Baojie Wang, Mei Ding, Hao Pang

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Article in Journal of clinical laboratory analysis, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

What it found

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Enzhu JiangSchool of Forensic Medicine, China Medical University, Shenyang, P.R. China.
Fengrui LiSchool of Forensic Medicine, China Medical University, Shenyang, P.R. China.
Chenchen JingSchool of Forensic Medicine, China Medical University, Shenyang, P.R. China.
Pei LiSchool of Forensic Medicine, China Medical University, Shenyang, P.R. China.
Honggang CuiSchool of Forensic Medicine, China Medical University, Shenyang, P.R. China.
Baojie WangSchool of Forensic Medicine, China Medical University, Shenyang, P.R. China.
Mei DingSchool of Forensic Medicine, China Medical University, Shenyang, P.R. China.
Hao PangSchool of Forensic Medicine, China Medical University, Shenyang, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSingle-nucleotide polymorphisms (SNPs) have been reported as a highly relevant point for the mechanisms of Parkinson's disease (PD). The invention of saturating dye makes it possible to identify heteroduplex DNA without redistribution during melting, which allows using high-resolution melting (HRM) to detect SNPs. However, the HRM analysis for detection of those SNPs associated with PD was rarely applied.

methodsTwo SNPs, G2385R and R1628P, located in leucine-rich repeat kinase 2 (LRRK2) gene were individually and multiplexedly genotyped using HRM analysis. The sequence variant observed in unexpected HRM curves was confirmed by DNA sequencing.

resultsHRM analysis identified successfully all genotypes both on R1628P and G2385R loci. The unexpected HRM curves appeared in R1628P amplicon generated from combinations of R1628P and rs11176013 loci. A multiplexed HRM assay that genotyped R1628P, rs11176013, and G2385R loci was efficiently established.

conclusionsThe present HRM assay is a reliable and rapid method for genotyping R1628P and G2385R loci in LRRK2 gene, and multiplex HRM analysis results in high throughput and has the potential to facilitate a wide range of genotyping studies on PD susceptibility genes.

Indexed as

Genetic LociGenetic Predisposition to DiseaseNucleic Acid DenaturationGenotyping TechniquesHumansLeucine-Rich Repeat Serine-Threonine Protein Kinase-2MutationParkinson DiseaseProtein Serine-Threonine KinasesLeucine-Rich Repeat Serine-Threonine Protein Kinase-2LRRK2 protein, humanProtein Serine-Threonine KinasesgenotypingHRMLRRK2Parkinson's diseaseSNPssusceptibility locus

Identifiers

PMID24849765
PMCPMC6807008

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