ArticleACS chemical neuroscience2014
Optogenetic control of serotonin and dopamine release in Drosophila larvae.
Article in ACS chemical neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- An opto-microperfusion neural probe for multiplexed detection of optogenetically evoked neurochemical release dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Engineering Protein Stability with Small Molecules: A Review of the ecDHFR Destabilizing Domain System.ACS chemical biology · 2026Review
- A pair of dopaminergic neurons DAN-c1 mediateeLife · 2025Article
- Electrochemical and biosensor techniques to monitor neurotransmitter changes with depression.Analytical and bioanalytical chemistry · 2024Review
- Metadynamics simulations reveal mechanisms of Na+ and Ca2+ transport in two open states of the channelrhodopsin chimera, C1C2.PloS one · 2024Article
- Understanding Neuropeptide Transmission in the Brain by Optical Uncaging and Release.ACS chemical neuroscience · 2023Review
- Significance of an Electrochemical Sensor and Nanocomposites: Toward the Electrocatalytic Detection of Neurotransmitters and Their Importance within the Physiological System.ACS nanoscience Au · 2023Review
- Article
- SSRI antidepressants differentially modulate serotonin reuptake and release in Drosophila.Journal of neurochemistry · 2022Article
- Effectiveness and Relationship between Biased and Unbiased Measures of Dopamine Release and Clearance.ACS chemical neuroscience · 2022Article
- Ring Finger Protein 11 (RNF11) Modulates Dopamine Release in Drosophila.Neuroscience · 2021Article
- Improving serotonin fast-scan cyclic voltammetry detection: new waveforms to reduce electrode fouling.The Analyst · 2020Article
- Real-Time Measurement of Stimulated Dopamine Release in Compartments of the AdultAnalytical chemistry · 2020Article
- Cavity Carbon-Nanopipette Electrodes for Dopamine Detection.Analytical chemistry · 2019Article
- Drosophila as a Model System for Neurotransmitter Measurements.ACS chemical neuroscience · 2018Review
- The Protective Action Encoding of Serotonin Transients in the Human Brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Article
- Functional Imaging and Optogenetics inGenetics · 2018Review
- Nicotinic acetylcholine receptor (nAChR) mediated dopamine release in larval Drosophila melanogaster.Neurochemistry international · 2018Article
- Fast-Scan Cyclic Voltammetry (FSCV) Detection of Endogenous Octopamine in Drosophila melanogaster Ventral Nerve Cord.ACS chemical neuroscience · 2016Article
- Modulatory Action by the Serotonergic System: Behavior and Neurophysiology in Drosophila melanogaster.Neural plasticity · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Optogenetic control of neurotransmitter release is an elegant method to investigate neurobiological mechanisms with millisecond precision and cell type-specific resolution. Channelrhodopsin-2 (ChR2) can be expressed in specific neurons, and blue light used to activate those neurons. Previously, in Drosophila, neurotransmitter release and uptake have been studied after continuous optical illumination. In this study, we investigated the effects of pulsed optical stimulation trains on serotonin or dopamine release in larval ventral nerve cords. In larvae with ChR2 expressed in serotonergic neurons, low-frequency stimulations produced a distinct, steady-state response while high-frequency patterns were peak shaped. Evoked serotonin release increased with increasing stimulation frequency and then plateaued. The steady-state response and the frequency dependence disappeared after administering the uptake inhibitor fluoxetine, indicating that uptake plays a significant role in regulating the extracellular serotonin concentration. Pulsed stimulations were also used to evoke dopamine release in flies expressing ChR2 in dopaminergic neurons and similar frequency dependence was observed. Release due to pulsed optical stimulations was modeled to determine the uptake kinetics. For serotonin, Vmax was 0.54 ± 0.07 μM/s and Km was 0.61 ± 0.04 μM; and for dopamine, Vmax was 0.12 ± 0.03 μM/s and Km was 0.45 ± 0.13 μM. The amount of serotonin released per stimulation pulse was 4.4 ± 1.0 nM, and the amount of dopamine was 1.6 ± 0.3 nM. Thus, pulsed optical stimulations can be used to mimic neuronal firing patterns and will allow Drosophila to be used as a model system for studying mechanisms underlying neurotransmission.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.