ArticleThe Journal of biological chemistry2014
Structural and evolutionary aspects of antenna chromophore usage by class II photolyases.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Role of photolyase in stress tolerance and virulence of plant-pathogenic bacteriumApplied and environmental microbiology · 2026Article
- Article
- Redox-State-Dependent Structural Changes within a Prokaryotic 6-4 Photolyase.Journal of the American Chemical Society · 2025Article
- Dynamics and mechanism of DNA repair by a bifunctional cryptochrome.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Deazaflavin metabolite produced by endosymbiotic bacteria controls fungal host reproduction.The ISME journal · 2024Article
- A structural decryption of cryptochromes.Frontiers in chemistry · 2024Review
- Identification and characterization of a prokaryotic 6-4 photolyase from Synechococcus elongatus with a deazariboflavin antenna chromophore.Nucleic acids research · 2022Article
- Cofactor F420: an expanded view of its distribution, biosynthesis and roles in bacteria and archaea.FEMS microbiology reviews · 2021Article
- A topologically distinct class of photolyases specific for UV lesions within single-stranded DNA.Nucleic acids research · 2020Article
- Review
- A Case Study of Eukaryogenesis: The Evolution of Photoreception by Photolyase/Cryptochrome Proteins.Journal of molecular evolution · 2020Article
- Enhanced DNA repair by DNA photolyase bearing an artificial light-harvesting chromophore.Nucleic acids research · 2020Article
- Deazaflavin reductive photocatalysis involves excited semiquinone radicals.Nature communications · 2020Article
- Chemical and structural analysis of a photoactive vertebrate cryptochrome from pigeon.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Structure of the bifunctional cryptochrome aCRY from Chlamydomonas reinhardtii.Nucleic acids research · 2018Article
- Article
- Light-controlled motility in prokaryotes and the problem of directional light perception.FEMS microbiology reviews · 2017Review
- Photolyase: Dynamics and electron-transfer mechanisms of DNA repair.Archives of biochemistry and biophysics · 2017Review
- Protecting DNA from errors and damage: an overview of DNA repair mechanisms in plants compared to mammals.Cellular and molecular life sciences : CMLS · 2017Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light-harvesting and resonance energy transfer to the catalytic FAD cofactor are key roles for the antenna chromophores of light-driven DNA photolyases, which remove UV-induced DNA lesions. So far, five chemically diverse chromophores have been described for several photolyases and related cryptochromes, but no correlation between phylogeny and used antenna has been found. Despite a common protein topology, structural analysis of the distantly related class II photolyase from the archaeon Methanosarcina mazei (MmCPDII) as well as plantal orthologues indicated several differences in terms of DNA and FAD binding and electron transfer pathways. For MmCPDII we identify 8-hydroxydeazaflavin (8-HDF) as cognate antenna by in vitro and in vivo reconstitution, whereas the higher plant class II photolyase from Arabidopsis thaliana fails to bind any of the known chromophores. According to the 1.9 Å structure of the MmCPDII·8-HDF complex, its antenna binding site differs from other members of the photolyase-cryptochrome superfamily by an antenna loop that changes its conformation by 12 Å upon 8-HDF binding. Additionally, so-called N- and C-motifs contribute as conserved elements to the binding of deprotonated 8-HDF and allow predicting 8-HDF binding for most of the class II photolyases in the whole phylome. The 8-HDF antenna is used throughout the viridiplantae ranging from green microalgae to bryophyta and pteridophyta, i.e. mosses and ferns, but interestingly not in higher plants. Overall, we suggest that 8-hydroxydeazaflavin is a crucial factor for the survival of most higher eukaryotes which depend on class II photolyases to struggle with the genotoxic effects of solar UV exposure.
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