ArticleEndocrinology2014
Pseudomonas aeruginosa elastase disrupts the cortisol-binding activity of corticosteroid-binding globulin.
Article in Endocrinology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- [Factors and conditions affecting transcortin production and function in blood plasma].Problemy endokrinologii · 2025Review
- RCL glycosylation of serum corticosteroid-binding globulin: implications in cortisol delivery and septic shock.Glycobiology · 2025Article
- The NE/AAT/CBG axis regulates adipose tissue glucocorticoid exposure.Nature communications · 2025Article
- Binding for life: corticosteroid binding globulin from vertebrate physiology to human diseases.Frontiers in endocrinology · 2025Review
- Subversion of the Complement System by Pseudomonas aeruginosa.Journal of bacteriology · 2023Review
- Early life stress causes persistent impacts on the microbiome of Atlantic salmon.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2021Article
- Cortisol-Related Signatures of Stress in the Fish Microbiome.Frontiers in microbiology · 2020Article
- N-Glycosylation influences human corticosteroid-binding globulin measurements.Endocrine connections · 2019Article
- Neutrophil elastase-cleaved corticosteroid-binding globulin is absent in human plasma.The Journal of endocrinology · 2019Article
- Functional implications of corticosteroid-binding globulinJournal of molecular endocrinology · 2018Article
- Opposite Expression of Hepatic and Pulmonary Corticosteroid-Binding Globulin in Cystic Fibrosis Patients.Frontiers in pharmacology · 2018Article
- Treatable traits and therapeutic targets: Goals for systems biology in infectious disease.Current opinion in systems biology · 2017Review
- CRH Affects the Phenotypic Expression of Sepsis-Associated Virulence Factors byFrontiers in cellular and infection microbiology · 2017Article
- Asn347 Glycosylation of Corticosteroid-binding Globulin Fine-tunes the Host Immune Response by Modulating Proteolysis by Pseudomonas aeruginosa and Neutrophil Elastase.The Journal of biological chemistry · 2016Article
- Corticosteroid-binding globulin is a biomarker of inflammation onset and severity in female rats.The Journal of endocrinology · 2016Article
- Plasma steroid-binding proteins: primary gatekeepers of steroid hormone action.The Journal of endocrinology · 2016Review
- Identification of Avian Corticosteroid-binding Globulin (SerpinA6) Reveals the Molecular Basis of Evolutionary Adaptations in SerpinA6 Structure and Function as a Steroid-binding Protein.The Journal of biological chemistry · 2016Article
- Biomarker candidates for the detection of an infectious etiology of febrile neutropenia.Infection · 2016Article
- Structural characterization of zinc-bound Zmp1, a zinc-dependent metalloprotease secreted by Clostridium difficile.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2016Article
- Third-generation Mineralocorticoid Receptor Antagonists: Why Do We Need a Fourth?Journal of cardiovascular pharmacology · 2016Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The serine protease inhibitor (SERPIN) family member corticosteroid-binding globulin (CBG) is the main carrier of glucocorticoids in plasma. Human CBG mediates the targeted release of cortisol at sites of inflammation through cleavage of its reactive center loop (RCL) by neutrophil elastase. The RCLs of SERPIN family members are targeted by diverse endogenous and exogenous proteases, including several bacterial proteases. We tested different bacteria for their ability to secrete proteases that disrupt CBG cortisol-binding activity, and characterized the responsible protease and site of CBG cleavage. Serum CBG integrity was assessed by Western blotting and cortisol-binding capacity assay. Effects of time, pH, temperature, and protease inhibitors were tested. Proteolytically active proteins from bacterial media were purified by fast protein liquid chromatography, and the active protease and CBG cleavage sites were identified by mass spectrometry. Among the bacteria tested, medium from Pseudomonas aeruginosa actively disrupted the cortisol-binding activity of CBG. This proteolytic activity was inhibited by zinc chelators and occurred most efficiently at pH 7 and elevated physiological temperature (ie, 41°C). Mass spectrometric analysis of a semi-purified fraction of P. aeruginosa media identified the virulence factor LasB as the responsible protease, and this was confirmed by assaying media from LasB-deficient P. aeruginosa. This metalloprotease cleaves the CBG RCL at a major site, distinct from that targeted by neutrophil elastase. Our results suggest that humoral responses to P. aeruginosa infection are influenced by this pathogen's ability to secrete a protease that promotes the release of the anti-inflammatory steroid, cortisol, from its plasma transport protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.