ArticlePloS one2014
Overexpression of a defensin enhances resistance to a fruit-specific anthracnose fungus in pepper.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 60 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Bioinformatics Analysis of thePlants (Basel, Switzerland) · 2024Article
- Plant-Derived Antimicrobial Peptides: Novel Preservatives for the Food Industry.Foods (Basel, Switzerland) · 2022Review
- Integrative RNA-Seq analysis ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Spray-induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake.Plant biotechnology journal · 2021Article
- Transgenic and genome-edited fruits: background, constraints, benefits, and commercial opportunities.Horticulture research · 2021Review
- Article
- Potent Anti-Candida Fraction Isolated from Capsicum chinense Fruits Contains an Antimicrobial Peptide That is Similar to Plant Defensin and is Able to Inhibit the Activity of Different α-Amylase Enzymes.Probiotics and antimicrobial proteins · 2021Article
- Structural Diversity and Highly Specific Host-Pathogen Transcriptional Regulation of Defensin Genes Is Revealed in Tomato.International journal of molecular sciences · 2020Article
- Host-induced silencing of the Colletotrichum gloeosporioides conidial morphology 1 gene (CgCOM1) confers resistance against Anthracnose disease in chilli and tomato.Plant molecular biology · 2020Article
- Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi.Bioscience reports · 2020Article
- Flagellin of Bacillus amyloliquefaciens works as a resistance inducer against groundnut bud necrosis virus in chilli (Capsicum annuum L.).Archives of virology · 2020Article
- Field Resistance toFrontiers in plant science · 2020Article
- Article
- Characterization and antifungal activity of a plant peptide expressed in the interaction between Capsicum annuum fruits and the anthracnose fungus.Bioscience reports · 2019Article
- Article
- Article
- Antimicrobial Peptides from Fruits and Their Potential Use as Biotechnological Tools-A Review and Outlook.Frontiers in microbiology · 2016Review
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8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional characterization of a defensin, J1-1, was conducted to evaluate its biotechnological potentiality in transgenic pepper plants against the causal agent of anthracnose disease, Colletotrichum gloeosporioides. To determine antifungal activity, J1-1 recombinant protein was generated and tested for the activity against C. gloeosporioides, resulting in 50% inhibition of fungal growth at a protein concentration of 0.1 mg·mL-1. To develop transgenic pepper plants resistant to anthracnose disease, J1-1 cDNA under the control of 35S promoter was introduced into pepper via Agrobacterium-mediated genetic transformation method. Southern and Northern blot analyses confirmed that a single copy of the transgene in selected transgenic plants was normally expressed and also stably transmitted to subsequent generations. The insertion of T-DNA was further analyzed in three independent homozygous lines using inverse PCR, and confirmed the integration of transgene in non-coding region of genomic DNA. Immunoblot results showed that the level of J1-1 proteins, which was not normally accumulated in unripe fruits, accumulated high in transgenic plants but appeared to differ among transgenic lines. Moreover, the expression of jasmonic acid-biosynthetic genes and pathogenesis-related genes were up-regulated in the transgenic lines, which is co-related with the resistance of J1-1 transgenic plants to anthracnose disease. Consequently, the constitutive expression of J1-1 in transgenic pepper plants provided strong resistance to the anthracnose fungus that was associated with highly reduced lesion formation and fungal colonization. These results implied the significance of the antifungal protein, J1-1, as a useful agronomic trait to control fungal disease.
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