Evidence map›Paper›PMID 24848238›Full record

ArticleNature chemistry2014

Bioorthogonal cyclization-mediated in situ self-assembly of small-molecule probes for imaging caspase activity in vivo.

Deju Ye, Adam J Shuhendler, Lina Cui, Ling Tong, Sui Seng Tee, Grigory Tikhomirov, Dean W Felsher, Jianghong Rao

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.

0numbers the graph read from it
0cells of the map it votes in
125citing papers in PubMed
21.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

125 citing papers in PubMed, 483 citations in OpenAlex.

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65 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Deju Ye1] Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, Stanford, California 94305-5484, USA [2].
Adam J Shuhendler1] Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, Stanford, California 94305-5484, USA [2].ORCID 0000000169525217
Lina CuiMolecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, Stanford, California 94305-5484, USA.
Ling TongDepartments of Medicine and Pathology, Division of Oncology, School of Medicine Stanford University, Stanford, California, 94305-5151, USA.
Sui Seng TeeMolecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, Stanford, California 94305-5484, USA.
Grigory TikhomirovMolecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, Stanford, California 94305-5484, USA.
Dean W FelsherDepartments of Medicine and Pathology, Division of Oncology, School of Medicine Stanford University, Stanford, California, 94305-5151, USA.
Jianghong RaoMolecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, Stanford, California 94305-5484, USA.
Stanford Medicine · US

Funding

Small Animal ImagingP50CA114747 · NCI · STANFORD UNIVERSITY · PI GAMBHIR, SANJIV S · 2005 to 2014
$18.2M
Pilot ProjectsU54CA151459 · NCI · STANFORD UNIVERSITY · PI QUAKE, STEPHEN R · 2010 to 2014
$12.6M
Modeling and Predicting Therapeutic Resistance of CancerU01CA188383 · NCI · STANFORD UNIVERSITY · PI FELSHER, DEAN W, GAMBHIR, SANJIV S · 2014 to 2018
$3.2M
Mechanisms by which Oncogene Inactivation Elicits Tumor Cell DeathR01CA170378 · NCI · STANFORD UNIVERSITY · PI FELSHER, DEAN W · 2012 to 2015
$2.1M
NCI NIH HHS 1P50CA114747-06NCI NIH HHS 1U54CA151459-01NCI NIH HHS P50 CA114747NCI NIH HHS R01 CA170378NCI NIH HHS U01 CA188383NCI NIH HHS U54 CA151459
6 · The paper itself

Abstract

Directed self-assembly of small molecules in living systems could enable a myriad of applications in biology and medicine, and already this has been used widely to synthesize supramolecules and nano/microstructures in solution and in living cells. However, controlling the self-assembly of synthetic small molecules in living animals is challenging because of the complex and dynamic in vivo physiological environment. Here we employ an optimized first-order bioorthogonal cyclization reaction to control the self-assembly of a fluorescent small molecule, and demonstrate its in vivo applicability by imaging caspase-3/7 activity in human tumour xenograft mouse models of chemotherapy. The fluorescent nanoparticles assembled in situ were imaged successfully in both apoptotic cells and tumour tissues using three-dimensional structured illumination microscopy. This strategy combines the advantages offered by small molecules with those of nanomaterials and should find widespread use for non-invasive imaging of enzyme activity in vivo.

Indexed as

Fluorescent DyesNanoparticlesAnimalsApoptosisCaspasesCyclizationDoxycyclineHeLa CellsHumansImage Processing, Computer-AssistedMiceMicroscopy, FluorescenceNeoplasms, ExperimentalXenograft Model Antitumor AssaysCaspasesDoxycyclineFluorescent Dyes

Identifiers

PMID24848238
PMCPMC4031611
OpenAlexW1991945497

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.