ArticlePloS one2014
A broadly reactive one-step SYBR Green I real-time RT-PCR assay for rapid detection of murine norovirus.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Antiviral Role of Surface Layer Protein A (SlpA) ofPathogens (Basel, Switzerland) · 2026Article
- Glutamate Supplementation Ameliorated Growth Impairment and Intestinal Injury in High-Soya-Meal-FedAnimals : an open access journal from MDPI · 2025Article
- Rodent Pathogen Detection via Testing of Soiled Nesting Material.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Toll-like receptor 7 protects against intestinal inflammation and restricts the development of colonic tissue-resident memory CD8Frontiers in immunology · 2024Article
- Amino acid substitutions in norovirus VP1 dictate host dissemination via variations in cellular attachment.Journal of virology · 2023Article
- Dietary High Glycinin Reduces Growth Performance and Impairs Liver and Intestinal Health Status of Orange-Spotted Grouper (Animals : an open access journal from MDPI · 2023Article
- Dietary Lactoferrin Supplementation Improves Growth Performance and Intestinal Health of Juvenile Orange-Spotted Groupers (Metabolites · 2022Article
- Development and Characterization of SYBR Green I Based RT-PCR Assay for Detection of Omsk Hemorrhagic Fever Virus.Virologica Sinica · 2021Article
- The Long Noncoding RNA ZFAS1 Potentiates the Development of Hepatocellular Carcinoma via the microRNA-624/MDK/ERK/JNK/P38 Signaling Pathway.OncoTargets and therapy · 2020Article
- LncRNA MINCR regulates irradiation resistance in nasopharyngeal carcinoma cells via the microRNA-223/ZEB1 axis.Cell cycle (Georgetown, Tex.) · 2020Article
- A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples.Experimental animals · 2019Article
- Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus.BMC veterinary research · 2018Article
- Murine norovirus infection in Brazilian animal facilities.Experimental animals · 2017Article
- Histologic Lesions Induced by Murine Norovirus Infection in Laboratory Mice.Veterinary pathology · 2016Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A one-step SYBR Green I real-time RT-PCR assay was developed for the detection and quantification of a broad range of murine noroviruses (MNVs). The primer design was based on the multiple sequence alignments of 101 sequences of the open reading frame (ORF)1-ORF2 junction of MNV. The broad reactivity and quantitative capacity of the assay were validated using 7 MNV plasmids. The assay was completed within 1 h, and the reliable detection limit was 10 copies of MNV plasmid or 0.063 median tissue culture infective doses per milliliter of RAW264 cell culture-propagated viruses. The diagnostic performance of the assay was evaluated using 158 mouse fecal samples, 91 of which were confirmed to be positive. The melting curve analysis demonstrated the diversity of MNV in the samples. This is the first report of a broadly reactive one-step SYBR Green I real-time RT-PCR assay for detecting of MNVs. The rapid and sensitive performance of this assay makes it a powerful tool for diagnostic applications.
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