ArticlePloS one2014
Transposon mutagenesis of the plant-associated Bacillus amyloliquefaciens ssp. plantarum FZB42 revealed that the nfrA and RBAM17410 genes are involved in plant-microbe-interactions.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 40 citations in OpenAlex.
- Review
- TheInternational journal of molecular sciences · 2023Article
- Plant-Associated Representatives of theMicroorganisms · 2023Article
- Novel Plant-AssociatedMicroorganisms · 2023Article
- Two plant-associatedFrontiers in plant science · 2023Article
- Plant-Microbe Interaction: Aboveground to Belowground, from the Good to the Bad.International journal of molecular sciences · 2021Review
- Inhibitory Abilities ofThe plant pathology journal · 2019Article
- Biofilm formation is determinant in tomato rhizosphere colonization by Bacillus velezensis FZB42.Environmental science and pollution research international · 2018Article
- Efficacy of Bacillus amyloliquefaciens as biocontrol agent to fight fungal diseases of maize under tropical climates: from lab to field assays in south Kivu.Environmental science and pollution research international · 2018Article
- Transcriptomic profiling of microbe-microbe interactions reveals the specific response of the biocontrol strain P. fluorescens In5 to the phytopathogen Rhizoctonia solani.BMC research notes · 2017Article
- Biocontrol Potentials of Antimicrobial Peptide ProducingFrontiers in microbiology · 2017Article
- Plant Microbe Interactions in Post Genomic Era: Perspectives and Applications.Frontiers in microbiology · 2016Review
- Understanding and engineering beneficial plant-microbe interactions: plant growth promotion in energy crops.Plant biotechnology journal · 2014Review
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11 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Bacillus amyloliquefaciens ssp. plantarum FZB42 represents the prototype of Gram-positive plant growth promoting and biocontrol bacteria. In this study, we applied transposon mutagenesis to generate a transposon library, which was screened for genes involved in multicellular behavior and biofilm formation on roots as a prerequisite of plant growth promoting activity. Transposon insertion sites were determined by rescue-cloning followed by DNA sequencing. As in B. subtilis, the global transcriptional regulator DegU was identified as an activator of genes necessary for swarming and biofilm formation, and the DegU-mutant of FZB42 was found impaired in efficient root colonization. Direct screening of 3,000 transposon insertion mutants for plant-growth-promotion revealed the gene products of nfrA and RBAM_017140 to be essential for beneficial effects exerted by FZB42 on plants. We analyzed the performance of GFP-labeled wild-type and transposon mutants in the colonization of lettuce roots using confocal laser scanning microscopy. While the wild-type strain heavily colonized root surfaces, the nfrA mutant did not colonize lettuce roots, although it was not impaired in growth in laboratory cultures, biofilm formation and swarming motility on agar plates. The RBAM17410 gene, occurring in only a few members of the B. subtilis species complex, was directly involved in plant growth promotion. None of the mutant strains were affected in producing the plant growth hormone auxin. We hypothesize that the nfrA gene product is essential for overcoming the stress caused by plant response towards bacterial root colonization.
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