ArticleACS synthetic biology2015
Fine-tuning interaction between aminoacyl-tRNA synthetase and tRNA for efficient synthesis of proteins containing unnatural amino acids.
Article in ACS synthetic biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Reaching New Heights in Genetic Code Manipulation with High Throughput Screening.Chemical reviews · 2024Review
- Minimizing the Anticodon-Recognized Loop ofBiomolecules · 2023Article
- Proximity-enhanced protein crosslinking through an alkene-tetrazine reaction.Bioorganic chemistry · 2023Article
- Impact of queuosine modification of endogenousFrontiers in molecular biosciences · 2022Article
- Biochemistry of Aminoacyl tRNA Synthetase and tRNAs and Their Engineering for Cell-Free and Synthetic Cell Applications.Frontiers in bioengineering and biotechnology · 2022Review
- Directed Evolution Pipeline for the Improvement of Orthogonal Translation Machinery for Genetic Code Expansion at Sense Codons.Frontiers in chemistry · 2022Article
- Directed-evolution of translation system for efficient unnatural amino acids incorporation and generalizable synthetic auxotroph construction.Nature communications · 2021Article
- Engineering of a Small Protein Scaffold To Recognize Sulfotyrosine with High Specificity.ACS chemical biology · 2021Article
- Review
- Exploring protein phosphorylation by combining computational approaches and biochemical methods.Computational and structural biotechnology journal · 2020Article
- Site-Specific Incorporation of Unnatural Amino Acids into Escherichia coli Recombinant Protein: Methodology Development and Recent Achievement.Biomolecules · 2019Review
- Genetic Incorporation of Noncanonical Amino Acids Using Two Mutually Orthogonal Quadruplet Codons.ACS synthetic biology · 2019Article
- A genetically encoded cyclobutene probe for labelling of live cells.Chemical communications (Cambridge, England) · 2017Article
- Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins.Biotechnology and bioengineering · 2017Article
- Synthetic biology approach for the development of conditionally replicating HIV-1 vaccine.Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) · 2017Article
- Fluorogenic protein labeling using a genetically encoded unstrained alkene.Chemical science · 2017Article
- Expanding the genetic code of Salmonella with non-canonical amino acids.Scientific reports · 2016Article
- Improved Incorporation of Noncanonical Amino Acids by an Engineered tRNA(Tyr) Suppressor.Biochemistry · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
By using a directed evolution approach, we have identified aminoacyl-tRNA synthetase variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to the amber nonsense codon in bacteria. We demonstrated that the optimization of anticodon recognition of tRNA by aminoacyl-tRNA synthetase led to improved incorporation efficiency that is unnatural amino acid-specific. The findings will facilitate the creation of an optimized system for the genetic incorporation of unnatural amino acids in bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.