Evidence map›Paper›PMID 24846008›Full record

ArticlePloS one2014

Mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) induce immune modulatory profile in monocyte-derived dendritic cells.

Fernando de Sá Silva, Rodrigo Nalio Ramos, Danilo Candido de Almeida, Enio Jose Bassi, Roberto Pereira Gonzales, Sueli Patricia Harumi Miyagi, Claudinéia Pereira Maranduba, Osvaldo Augusto Brazil Esteves Sant'Anna, Márcia Martins Marques, José Alexandre Marzagão Barbuto and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 57 citations in OpenAlex.

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  4. Mesenchymal Stem Cells Suppress Dendritic Cells and Modulate Proinflammatory Milieu Through Interleukin-10 Expression in Peripheral Blood Mononuclear Cells of Human Systemic Lupus Erythematosus.Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Fernando de Sá SilvaBiomedical Science Institute, University of São Paulo, São Paulo, Brazil; Dentistry Department, University of São Paulo, São Paulo, Brazil; Biomedical Science Institute, University of Juiz de Fora, Juiz de Fora, Brazil.
Rodrigo Nalio RamosBiomedical Science Institute, University of São Paulo, São Paulo, Brazil.
Danilo Candido de AlmeidaBiomedical Science Institute, University of São Paulo, São Paulo, Brazil.
Enio Jose BassiBiomedical Science Institute, University of São Paulo, São Paulo, Brazil.
Roberto Pereira GonzalesBiomedical Science Institute, University of São Paulo, São Paulo, Brazil.
Sueli Patricia Harumi MiyagiDentistry Department, University of São Paulo, São Paulo, Brazil.
Claudinéia Pereira MarandubaBiomedical Science Institute, University of Juiz de Fora, Juiz de Fora, Brazil.
Osvaldo Augusto Brazil Esteves Sant'AnnaBiomedical Science Institute, University of Juiz de Fora, Juiz de Fora, Brazil.
Márcia Martins MarquesImmunochemistry Laboratory, Butantan Institute, São Paulo, Brazil.
José Alexandre Marzagão BarbutoBiomedical Science Institute, University of São Paulo, São Paulo, Brazil.
Niels Olsen Saraiva CâmaraBiomedical Science Institute, University of São Paulo, São Paulo, Brazil.
Carlos Magno da Costa MarandubaBiomedical Science Institute, University of Juiz de Fora, Juiz de Fora, Brazil.
Universidade de São Paulo · BRUniversidade Federal de Juiz de Fora · BRInstituto Butantan · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMesenchymal stem cells have prominent immune modulatory properties, which may have clinical applications; however their major source, bone marrow, is of limited availability. On the other hand, mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) are readily accessible, but their immune regulatory properties have not been completely investigated. This study was designed, therefore, to evaluate the SHEDs influence on DCs differentiation, maturation, ability to activate T cells and to expand CD4(+)Foxp3(+) T cells. METHODOLOGY/PRINCIPAL

findingsThe experiments were based in cellular co-culture during differentiation and maturation of monocyte derived-DCs (moDCs), with, or not, presence of SHEDs. After co-culture with SHEDs, (moDCs) presented lower expression of BDCA-1 and CD11c, in comparison to DC cultivated without SHEDs. CD40, CD80, CD83 and CD86 levels were also decreased in mature DCs (mDCs) after co-cultivation with SHEDs. To assess the ability of SHEDs-exposed moDCs to modulate T cell responses, the former were separated from SHEDs, and co-cultured with peripheral blood lymphocytes. After 5 days, the proliferation of CD4(+) and CD8(+) T cells was evaluated and found to be lower than that induced by moDCs cultivated without SHEDs. In addition, an increase in the proportion of CD4(+)Foxp3(+)IL-10(+) T cells was observed among cells stimulated by mature moDCs that were previously cultivated with SHEDs. Soluble factors released during co-cultures also showed a reduction in the pro-inflammatory cytokines (IL-2, TNF-α and IFN-γ), and an increase in the anti-inflammatory molecule IL-10. CONCLUSION/SIGNIFICANCE: This study shows that SHEDs induce an immune regulatory phenotype in moDCs cells, evidenced by changes in maturation and differentiation rates, inhibition of lymphocyte stimulation and ability to expand CD4(+)Foxp3(+) T cells. Further characterization and validation of this phenomenon could support the use of SHEDs, directly or indirectly for immune modulation in the clinical practice.

Indexed as

Antigens, CD1BiomarkersCD40 AntigensCell DifferentiationCoculture TechniquesCytokinesDendritic CellsForkhead Transcription FactorsGlycoproteinsHumansImmunomodulationInterleukin-10Lymphocyte ActivationMesenchymal Stem CellsMonocytesPhenotypeAntigens, CD1BiomarkersCD1C protein, humanCD40 AntigensCytokinesForkhead Transcription FactorsFOXP3 protein, humanGlycoproteinsInterleukin-10

Identifiers

PMID24846008
PMCPMC4028272
OpenAlexW2027973244

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.