Evidence map›Paper›PMID 24845399›Full record

ArticlePloS one2014

Expressions of tight junction proteins Occludin and Claudin-1 are under the circadian control in the mouse large intestine: implications in intestinal permeability and susceptibility to colitis.

Oh-oka Kyoko, Hiroshi Kono, Kayoko Ishimaru, Kunio Miyake, Takeo Kubota, Hideoki Ogawa, Ko Okumura, Shigenobu Shibata, Atsuhito Nakao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.

0numbers the graph read from it
0cells of the map it votes in
103citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

103 citing papers in PubMed, 158 citations in OpenAlex.

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43 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Oh-oka KyokoDepartment of Immunology, University of Yamanashi Faculty of Medicine, Yamanashi, Japan.
Hiroshi KonoThe First Department of Surgery, University of Yamanashi Faculty of Medicine, Yamanashi, Japan.
Kayoko IshimaruDepartment of Immunology, University of Yamanashi Faculty of Medicine, Yamanashi, Japan.
Kunio MiyakeDepartment of Epigenetic Medicine, University of Yamanashi Faculty of Medicine, Yamanashi, Japan.
Takeo KubotaDepartment of Epigenetic Medicine, University of Yamanashi Faculty of Medicine, Yamanashi, Japan.
Hideoki OgawaAtopy Research Center, Juntendo University School of Medicine, Tokyo, Japan.
Ko OkumuraAtopy Research Center, Juntendo University School of Medicine, Tokyo, Japan.
Shigenobu ShibataDepartment of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Atsuhito NakaoDepartment of Immunology, University of Yamanashi Faculty of Medicine, Yamanashi, Japan; Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan.
University of Yamanashi · JPJuntendo University · JPWaseda University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND &

aimsThe circadian clock drives daily rhythms in behavior and physiology. A recent study suggests that intestinal permeability is also under control of the circadian clock. However, the precise mechanisms remain largely unknown. Because intestinal permeability depends on tight junction (TJ) that regulates the epithelial paracellular pathway, this study investigated whether the circadian clock regulates the expression levels of TJ proteins in the intestine.

methodsThe expression levels of TJ proteins in the large intestinal epithelium and colonic permeability were analyzed every 4, 6, or 12 hours between wild-type mice and mice with a mutation of a key clock gene Period2 (Per2; mPer2(m/m). In addition, the susceptibility to dextran sodium sulfate (DSS)-induced colitis was compared between wild-type mice and mPer2(m/m) mice.

resultsThe mRNA and protein expression levels of Occludin and Claudin-1 exhibited daily variations in the colonic epithelium in wild-type mice, whereas they were constitutively high in mPer2(m/m) mice. Colonic permeability in wild-type mice exhibited daily variations, which was inversely associated with the expression levels of Occludin and Claudin-1 proteins, whereas it was constitutively low in mPer2(m/m) mice. mPer2(m/m) mice were more resistant to the colonic injury induced by DSS than wild-type mice.

conclusionsOccludin and Claudin-1 expressions in the large intestine are under the circadian control, which is associated with temporal regulation of colonic permeability and also susceptibility to colitis.

Indexed as

Gene Expression RegulationAnimalsARNTL Transcription FactorsCircadian RhythmClaudin-1CLOCK ProteinsColitisE-Box ElementsFemaleGenetic Predisposition to DiseaseInterleukin-1betaIntestinal MucosaIntestine, LargeMiceMice, KnockoutOccludinARNTL Transcription FactorsClaudin-1CLOCK ProteinsInterleukin-1betaOccludinPer2 protein, mousePeriod Circadian ProteinsRNA, MessengerTumor Necrosis Factor-alpha

Identifiers

PMID24845399
PMCPMC4028230
OpenAlexW1987167904

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.