ArticlePloS one2014
An investigation of the diversity of strains of enteroaggregative Escherichia coli isolated from cases associated with a large multi-pathogen foodborne outbreak in the UK.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- EnteroaggregativeEcoSal Plus · 2025Review
- Microbiology and epidemiology of enteroaggregativeJournal of medical microbiology · 2025Article
- Molecular-based evidence for school transmission of enteroaggregative Escherichia coli among apparently healthy children attending nursery, infant, and primary schools in Madrid (Spain).European journal of pediatrics · 2025Article
- Genetic and phenotypic analysis of the virulence plasmid of a non-Shigatoxigenic enteroaggregativeMicrobiology (Reading, England) · 2025Article
- Manipulation of magnetic beads for actively capturingBiomicrofluidics · 2024Article
- Virulence Factors and Antimicrobial Resistance of UropathogenicBioMed research international · 2023Article
- To kill or to be killed: pangenome analysis of Escherichia coli strains reveals a tailocin specific for pandemic ST131.BMC biology · 2022Article
- Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study.International journal of molecular sciences · 2020Article
- Article
- Microbiological quality of raw drinking milk and unpasteurised dairy products: results from England 2013-2019.Epidemiology and infection · 2020Article
- Shiga Toxin-Producing and Enteroaggregative Escherichia coli in Animal, Foods, and Humans: Pathogenicity Mechanisms, Detection Methods, and Epidemiology.Current microbiology · 2020Review
- Novel type of pilus associated with a Shiga-toxigenic E. coli hybrid pathovar conveys aggregative adherence and bacterial virulence.Emerging microbes & infections · 2018Article
- Large outbreak of multiple gastrointestinal pathogens associated with fresh curry leaves in North East England, 2013.Epidemiology and infection · 2018Article
- Setup, Validation, and Quality Control of a Centralized Whole-Genome-Sequencing Laboratory: Lessons Learned.Journal of clinical microbiology · 2018Article
- Whole genome sequencing reveals high clonal diversity ofAntimicrobial resistance and infection control · 2018Article
- Abundant production of exopolysaccharide by EAEC strains enhances the formation of bacterial biofilms in contaminated sprouts.Gut microbes · 2018Article
- The wax moth Galleria mellonella as a novel model system to study Enteroaggregative Escherichia coli pathogenesis.Virulence · 2017Article
- Clonality, virulence and antimicrobial resistance of enteroaggregative Escherichia coli from Mirzapur, Bangladesh.Journal of medical microbiology · 2017Article
- Seeding and Establishment of Legionella pneumophila in Hospitals: Implications for Genomic Investigations of Nosocomial Legionnaires' Disease.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
- Evaluation of an Optimal Epidemiological Typing Scheme for Legionella pneumophila with Whole-Genome Sequence Data Using Validation Guidelines.Journal of clinical microbiology · 2016Article
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12 authors.
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Abstract
Following a large outbreak of foodborne gastrointestinal (GI) disease, a multiplex PCR approach was used retrospectively to investigate faecal specimens from 88 of the 413 reported cases. Gene targets from a range of bacterial GI pathogens were detected, including Salmonella species, Shigella species and Shiga toxin-producing Escherichia coli, with the majority (75%) of faecal specimens being PCR positive for aggR associated with the Enteroaggregative E. coli (EAEC) group. The 20 isolates of EAEC recovered from the outbreak specimens exhibited a range of serotypes, the most frequent being O104:H4 and O131:H27. None of the EAEC isolates had the Shiga toxin (stx) genes. Multilocus sequence typing and single nucleotide polymorphism analysis of the core genome confirmed the diverse phylogeny of the strains. The analysis also revealed a close phylogenetic relationship between the EAEC O104:H4 strains in this outbreak and the strain of E. coli O104:H4 associated with a large outbreak of haemolytic ureamic syndrome in Germany in 2011. Further analysis of the EAEC plasmids, encoding the key enteroaggregative virulence genes, showed diversity with respect to FIB/FII type, gene content and genomic architecture. Known EAEC virulence genes, such as aggR, aat and aap, were present in all but one of the strains. A variety of fimbrial genes were observed, including genes encoding all five known fimbrial types, AAF/1 to AAF/V. The AAI operon was present in its entirety in 15 of the EAEC strains, absent in three and present, but incomplete, in two isolates. EAEC is known to be a diverse pathotype and this study demonstrates that a high level of diversity in strains recovered from cases associated with a single outbreak. Although the EAEC in this study did not carry the stx genes, this outbreak provides further evidence of the pathogenic potential of the EAEC O104:H4 serotype.
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