Evidence map›Paper›PMID 24843546›Full record

ArticleJournal of diabetes investigation2012

Sorting nexin 19 regulates the number of dense core vesicles in pancreatic β-cells.

Shin-Ichi Harashima, Takahiko Horiuchi, Yu Wang, Abner Louis Notkins, Yutaka Seino, Nobuya Inagaki

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of diabetes investigation, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 28 citations in OpenAlex.

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  17. Article
  18. The emerging role of sorting nexins in cardiovascular diseases.Clinical science (London, England : 1979) · 2019
    Review
  19. Discovery and Roles of ER-Endolysosomal Contact Sites in Disease.Advances in experimental medicine and biology · 2017
    Review
  20. Global genomic and transcriptomic analysis of human pancreatic islets reveals novel genes influencing glucose metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2014
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Shin-Ichi HarashimaDepartment of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, Kyoto.
Takahiko HoriuchiDepartment of Medicine and Biosystemic Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Yu WangDepartment of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, Kyoto.
Abner Louis NotkinsExperimental Medicine Section, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research (NIDCR), National Institutes of Health (NIH), Bethesda, MD, USA.
Yutaka SeinoDepartment of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, Kyoto.
Nobuya InagakiDepartment of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, Kyoto.
Kyoto University · JPKyushu University · JPNational Institutes of Health · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

unlabelledAims/Introduction:  Insulinoma-associated protein 2 (IA-2) regulates insulin secretion and the number of dense core vesicles (DCV). However, the mechanism of regulation of DCV number by IA-2 is unknown. We examined the effect of sorting nexin 19 (SNX19), an IA-2 interacting protein, on insulin secretion and the number of dense core vesicles (DCV). MATERIALS AND

methodsStable SNX19 knockdown (SNX19KD) MIN6, a mouse pancreatic β-cell line, and stable SNX19-reintroduced SNX19KD MIN6 were established. Quantification of DCV, and lysosomes was carried out using electron micrographs. The half-life of DCV was detected by pulse-chase experiment.

resultsInsulin secretion and content were decreased in stable SNX19KD MIN6 cells compared with those in control MIN6 cells. Electron micrographs showed that DCV number in SNX19KD cells was decreased by approximately 75% and that DCV size was decreased by approximately 40% compared with those in control cells, respectively. Furthermore, when SNX19 was reintroduced in SNX19KD cells, insulin content, insulin secretion and DCV number were increased. The half-life of DCV was decreased in SNX19KD cells, but was increased in SNX19KD cells in which SNX19 was reintroduced. The number of lysosomes and the activity of lysosome enzyme cathepsin D were increased by approximately threefold in SNX19KD cells compared with those in control cells. In contrast, they were decreased to approximately half to one-third in SNX19-reintroduced SNX19KD cells.

conclusionsSNX19 regulates the number of DCV and insulin content by stabilizing DCV in β-cells. (J Diabetes Invest, doi: 10.1111/j.2040-1124.2011.00138.x, 2012).

Indexed as

Dense core vesiclesInsulinoma‐associated protein 2Sorting nexin 19

Identifiers

PMID24843546
PMCPMC4014933
OpenAlexW1515085794

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.