ArticleProceedings of the National Academy of Sciences of the United States of America2014
Two-photon imaging of remyelination of spinal cord axons by engrafted neural precursor cells in a viral model of multiple sclerosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Cell replacement therapy with stem cells in multiple sclerosis, a systematic review.Human cell · 2024Pooled it
- Mapping of blood flow and slow speckle tissue dynamics using laser speckle contrast imaging.Biomedical optics express · 2025Article
- Cystatin F attenuates neuroinflammation and demyelination following murine coronavirus infection of the central nervous system.Journal of neuroinflammation · 2024Article
- Presentation of Human Neural Stem Cell Antigens Drives Regulatory T Cell Induction.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Viral mouse models used to study multiple sclerosis: past and present.Archives of virology · 2021Review
- Regulatory T cells suppress Th17 cell CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Regulatory T cells promote remyelination in the murine experimental autoimmune encephalomyelitis model of multiple sclerosis following human neural stem cell transplant.Neurobiology of disease · 2020Article
- Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo.BMB reports · 2020Review
- Review
- The microbiota protects from viral-induced neurologic damage through microglia-intrinsic TLR signaling.eLife · 2019Article
- Promoting remyelination through cell transplantation therapies in a model of viral-induced neurodegenerative disease.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Review
- Aberration correction considering curved sample surface shape for non-contact two-photon excitation microscopy with spatial light modulator.Scientific reports · 2018Article
- Stem Cells as Potential Targets of Polyphenols in Multiple Sclerosis and Alzheimer's Disease.BioMed research international · 2018Review
- Article
- In vivo multiphoton imaging of immune cell dynamics.Pflugers Archiv : European journal of physiology · 2016Review
- Scutellarin Alleviates Behavioral Deficits in a Mouse Model of Multiple Sclerosis, Possibly Through Protecting Neural Stem Cells.Journal of molecular neuroscience : MN · 2016Article
- Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells.Stem cells international · 2016Article
- Imaging Axonal Degeneration and Repair in Preclinical Animal Models of Multiple Sclerosis.Frontiers in immunology · 2016Review
- Sphingosine-1-phosphate receptor antagonism enhances proliferation and migration of engrafted neural progenitor cells in a model of viral-induced demyelination.The American journal of pathology · 2015Article
- Two-photon imaging of cellular dynamics in the mouse spinal cord.Journal of visualized experiments : JoVE · 2015Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
Neural precursor cells (NPCs) offer a promising approach for treating demyelinating diseases. However, the cellular dynamics that underlie transplanted NPC-mediated remyelination have not been described. Using two-photon imaging of a newly developed ventral spinal cord preparation and a viral model of demyelination, we describe the motility and intercellular interactions of transplanted mouse NPCs expressing green fluorescent protein (GFP) with damaged axons expressing yellow fluorescent protein (YFP). Our findings reveal focal axonal degeneration that occurs in the ventral side of the spinal cord within 1 wk following intracranial instillation with the neurotropic JHM strain of mouse hepatitis virus (JHMV). Axonal damage precedes extensive demyelination and is characterized by swelling along the length of the axon, loss of YFP signal, and transected appearance. NPCs engrafted into spinal cords of JHMV-infected mice exhibited diminished migration velocities and increased proliferation compared with transplanted cells in noninfected mice. NPCs preferentially accumulated within areas of axonal damage, initiated direct contact with axons, and subsequently expressed the myelin proteolipid protein gene, initiating remyelination. These findings indicate that NPCs transplanted into an inflammatory demyelinating microenvironment participate directly in therapeutic outcome through the wrapping of myelin around damaged neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.