ArticleProceedings of the National Academy of Sciences of the United States of America2014
Trapped translocation intermediates establish the route for export of capsular polysaccharides across Escherichia coli outer membranes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Growth stage-related capsular polysaccharide translocon Wza in Vibrio splendidus modifies phage vB_VspM_VS2 susceptibility.Communications biology · 2024Article
- Evolutionary genomic analyses of canineApplied and environmental microbiology · 2024Article
- Article
- The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase, Wzz, from enterobacterial common antigen biosynthesis form a complex.Open biology · 2023Article
- Bacterial Outer Membrane Polysaccharide Export (OPX) Proteins Occupy Three Structural Classes with Selective β-Barrel Porin Requirements for Polymer Secretion.Microbiology spectrum · 2022Article
- Article
- Bacterial Glycocalyx Integrity Impacts Tolerance ofBiomolecules · 2022Article
- Article
- The molecular basis of regulation of bacterial capsule assembly by Wzc.Nature communications · 2021Article
- Assembly and Maintenance of Lipids at the Bacterial Outer Membrane.Chemical reviews · 2021Review
- Insights into the structure of Escherichia coli outer membrane as the target for engineering microbial cell factories.Microbial cell factories · 2021Review
- Article
- Modulation of bacterial multicellularity via spatio-specific polysaccharide secretion.PLoS biology · 2020Article
- Structural and Functional Variation in Outer Membrane Polysaccharide Export (OPX) Proteins from the Two Major Capsule Assembly Pathways Present in Escherichia coli.Journal of bacteriology · 2019Article
- Structural features underlying recognition and translocation of extracellular polysaccharides.Interface focus · 2019Review
- Outer membrane lipoprotein RlpA is a novel periplasmic interaction partner of the cell division protein FtsK in Escherichia coli.Scientific reports · 2018Article
- Periplasmic depolymerase provides insight into ABC transporter-dependent secretion of bacterial capsular polysaccharides.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Synthesis of Rhizobial Exopolysaccharides and Their Importance for Symbiosis with Legume Plants.Genes · 2017Review
- Review
- Chemical polyglycosylation and nanolitre detection enables single-molecule recapitulation of bacterial sugar export.Nature chemistry · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The outer membrane (OM) of Gram-negative bacteria is designed to exclude potentially harmful molecules. This property presents a challenge for bacteria that must secrete proteins and large glycoconjugates to grow, divide, and persist. Proteins involved in trafficking such molecules have been identified, but their precise roles are often unresolved due to the difficulty in capturing "snapshots" during the export pathway. Wza is the prototype for the large family of OM polysaccharide export proteins. In Escherichia coli, Wza is essential for the assembly of a capsule, a protective surface coat composed of long-chain polysaccharides. Wza creates an octameric α-helical channel spanning the OM, but the bulk of the protein exists as a large periplasmic structure enclosing an extensive lumen. Residues within the lumen of Wza were targeted for site-specific incorporation of the UV photo-cross-linkable unnatural amino acid p-benzoyl-L-phenylalanine. Using this in vivo photo-cross-linking strategy, we were able to trap polysaccharide translocation intermediates within the lumen of Wza, providing the first unequivocal evidence to our knowledge that nascent capsular polysaccharide chains exit the cell through the Wza portal.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.