ArticleeLife2014
Symmetry breaking in reconstituted actin cortices.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 109 citations in OpenAlex.
- Reconstituted systems for studying the architecture and dynamics of actin networks.The Biochemical journal · 2025Review
- Density-dependent flow generation in active cytoskeletal fluids.Scientific reports · 2024Article
- Benefits and challenges of reconstituting the actin cortex.Cytoskeleton (Hoboken, N.J.) · 2024Review
- Supramolecular fibrillation in coacervates and other confined systems towards biomimetic function.Communications chemistry · 2024Review
- Synthetic control of actin polymerization and symmetry breaking in active protocells.Science advances · 2024Article
- Microrail-assisted liposome trapping and aligning in microfluidic channels.RSC advances · 2024Article
- Kinetic trapping organizes actin filaments within liquid-like protein droplets.Nature communications · 2024Article
- Unveiling the physics underlying symmetry breaking of the actin cytoskeleton: An artificial cell-based approach.Biophysics and physicobiology · 2023Article
- Geometric trade-off between contractile force and viscous drag determines the actomyosin-based motility of a cell-sized droplet.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy.Journal of visualized experiments : JoVE · 2022Article
- Orientation of Cell Polarity by Chemical Gradients.Annual review of biophysics · 2022Review
- Patterning Microtubule Network Organization Reshapes Cell-Like Compartments.ACS synthetic biology · 2021Article
- Dynamic self-assembly of compartmentalized DNA nanotubes.Nature communications · 2021Article
- Intra-bundle contractions enable extensile properties of active actin networks.Scientific reports · 2021Article
- Tug-of-war between actomyosin-driven antagonistic forces determines the positioning symmetry in cell-sized confinement.Nature communications · 2020Article
- Article
- A Cell Adhesion-Based Reconstitution Method for Studying Cell Polarity.Frontiers in cell and developmental biology · 2020Article
- Scaling behaviour in steady-state contracting actomyosin networks.Nature physics · 2019Article
- Article
- Cytoskeletal actin patterns shape mast cell activation.Communications biology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The actin cortex plays a pivotal role in cell division, in generating and maintaining cell polarity and in motility. In all these contexts, the cortical network has to break symmetry to generate polar cytoskeletal dynamics. Despite extensive research, the mechanisms responsible for regulating cortical dynamics in vivo and inducing symmetry breaking are still unclear. Here we introduce a reconstituted system that self-organizes into dynamic actin cortices at the inner interface of water-in-oil emulsions. This artificial system undergoes spontaneous symmetry breaking, driven by myosin-induced cortical actin flows, which appears remarkably similar to the initial polarization of the embryo in many species. Our in vitro model system recapitulates the rich dynamics of actin cortices in vivo, revealing the basic biophysical and biochemical requirements for cortex formation and symmetry breaking. Moreover, this synthetic system paves the way for further exploration of artificial cells towards the realization of minimal model systems that can move and divide.DOI: http://dx.doi.org/10.7554/eLife.01433.001.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.