ArticlePloS one2014
Apple derived cellulose scaffolds for 3D mammalian cell culture.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
72 citing papers in PubMed, 213 citations in OpenAlex.
- Growing Relevance of Decellularized Plant Material as Functional Scaffolds in Tissue Engineering and Cultured Meat: A Scoping Review on Recent Advances, Challenges, and Future Directions.Annals of biomedical engineering · 2026Review
- Application of 3D Cell Culture Techniques in Nanotoxicology: How Far Are We?Stem cell reviews and reports · 2026Review
- Cellulose Plant-Derived Scaffolds as a Tool for Myometrium Modeling.International journal of molecular sciences · 2025Article
- Autofluorescence Quenching in Decellularized Plant Scaffolds for Tissue Engineering.Annals of biomedical engineering · 2025Article
- Poly-L-Ornithine coated plant scaffolds support motor recovery in rats after traumatic spinal cord injury.Scientific reports · 2025Article
- Plant-Based Scaffolds for Tissue Engineering: A Review.Polymers · 2025Review
- Innovations, Challenges, and Regulatory Pathways in Cultured Meat for a Sustainable Future.Foods (Basel, Switzerland) · 2025Review
- Article
- Mechanical Properties and Microstructure of Decellularized Brown Seaweed Scaffold for Tissue Engineering.Bioengineering (Basel, Switzerland) · 2025Article
- Decellularized apple hypanthium as a plant-based biomaterial for cartilage regeneration in vitro: a comparative study of progenitor cell types and environmental conditions.Journal of biological engineering · 2025Article
- Cultivation of bovine lipid chunks on Aloe vera scaffolds.NPJ science of food · 2025Article
- Growing meat on autoclaved vegetables with biomimetic stiffness and micro-patterns.Nature communications · 2025Article
- Biocompatibility of decellularized spinach leaves.npj biomedical innovations · 2025Article
- Decellularized Green and Brown Macroalgae as Cellulose Matrices for Tissue Engineering.Journal of functional biomaterials · 2024Article
- Decellularized Macroalgae as Complex Hydrophilic Structures for Skin Tissue Engineering and Drug Delivery.Gels (Basel, Switzerland) · 2024Article
- From Nature to Technology: Exploring the Potential of Plant-Based Materials and Modified Plants in Biomimetics, Bionics, and Green Innovations.Biomimetics (Basel, Switzerland) · 2024Review
- Decellularised plant scaffolds facilitate porcine skeletal muscle tissue engineering for cultivated meat biomanufacturing.NPJ science of food · 2024Article
- Cultivation of Bovine Mesenchymal Stem Cells on Plant-Based Scaffolds in a Macrofluidic Single-Use Bioreactor for Cultured Meat.Foods (Basel, Switzerland) · 2024Article
- Application of pulsed electric field technology to skin engineering.Frontiers in bioengineering and biotechnology · 2024Review
- The important role of cellular mechanical microenvironment in engineering structured cultivated meat: Recent advances.Current research in food science · 2024Review
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There are numerous approaches for producing natural and synthetic 3D scaffolds that support the proliferation of mammalian cells. 3D scaffolds better represent the natural cellular microenvironment and have many potential applications in vitro and in vivo. Here, we demonstrate that 3D cellulose scaffolds produced by decellularizing apple hypanthium tissue can be employed for in vitro 3D culture of NIH3T3 fibroblasts, mouse C2C12 muscle myoblasts and human HeLa epithelial cells. We show that these cells can adhere, invade and proliferate in the cellulose scaffolds. In addition, biochemical functionalization or chemical cross-linking can be employed to control the surface biochemistry and/or mechanical properties of the scaffold. The cells retain high viability even after 12 continuous weeks of culture and can achieve cell densities comparable with other natural and synthetic scaffold materials. Apple derived cellulose scaffolds are easily produced, inexpensive and originate from a renewable source. Taken together, these results demonstrate that naturally derived cellulose scaffolds offer a complementary approach to existing techniques for the in vitro culture of mammalian cells in a 3D environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.