ArticleHepatology (Baltimore, Md.)2014
Shotgun proteomics: identification of unique protein profiles of apoptotic bodies from biliary epithelial cells.
Article in Hepatology (Baltimore, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 67 citations in OpenAlex.
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- Extracellular Vesicles' Role in the Pathophysiology and as Biomarkers in Cystic Fibrosis and COPD.International journal of molecular sciences · 2022Review
- Pathological Contribution of Extracellular Vesicles and Their MicroRNAs to Progression of Chronic Liver Disease.Biology · 2022Review
- The Therapeutic Role of ADSC-EVs in Skin Regeneration.Frontiers in medicine · 2022Review
- Circulating extracellular vesicles and rheumatoid arthritis: a proteomic analysis.Cellular and molecular life sciences : CMLS · 2021Review
- Emerging understanding of apoptosis in mediating mesenchymal stem cell therapy.Cell death & disease · 2021Review
- Combination of lyophilized adipose-derived stem cell concentrated conditioned medium and polysaccharide hydrogel in the inhibition of hypertrophic scarring.Stem cell research & therapy · 2021Article
- Primary Biliary Cholangitis and Bile Acid Farnesoid X Receptor Agonists.Diseases (Basel, Switzerland) · 2020Review
- Apoptosis and apoptotic body: disease message and therapeutic target potentials.Bioscience reports · 2019Review
- Moving beyond size and phosphatidylserine exposure: evidence for a diversity of apoptotic cell-derived extracellular vesiclesJournal of extracellular vesicles · 2019Article
- Detection and Isolation of Apoptotic Bodies to High Purity.Journal of visualized experiments : JoVE · 2018Article
- Extracellular Vesicle Biology in the Pathogenesis of Lung Disease.American journal of respiratory and critical care medicine · 2017Article
- Extracellular vesicles as emerging targets in cancer: Recent development from bench to bedside.Biochimica et biophysica acta. Reviews on cancer · 2017Review
- The modulation of co-stimulatory molecules by circulating exosomes in primary biliary cirrhosis.Cellular & molecular immunology · 2017Article
- Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.Scientific reports · 2017Article
- MUMAL2: Improving sensitivity in shotgun proteomics using cost sensitive artificial neural networks and a threshold selector algorithm.BMC bioinformatics · 2016Article
- Endogenous interleukin-22 protects against inflammatory bowel disease but not autoimmune cholangitis in dominant negative form of transforming growth factor beta receptor type II mice.Clinical and experimental immunology · 2016Article
- Adaptive immunity in the liver.Cellular & molecular immunology · 2016Review
- Deletion of Galectin-3 Enhances Xenobiotic Induced Murine Primary Biliary Cholangitis by Facilitating Apoptosis of BECs and Release of Autoantigens.Scientific reports · 2016Article
- Focus on Extracellular Vesicles: Introducing the Next Small Big Thing.International journal of molecular sciences · 2016Review
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 4 countries.
Funding
Abstract
unlabelledShotgun proteomics is a powerful analytic method to characterize complex protein mixtures in combination with multidimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS). We used this platform for proteomic characterization of apoptotic bodies in an effort to define the complex protein mixtures found in primary cultures of human intrahepatic biliary epithelial cells (HiBEC), human renal proximal tubular epithelial cells, human bronchial epithelial cells, isolated intrahepatic biliary epithelial cells from explanted primary biliary cirrhosis (PBC), and control liver using a total of 24 individual samples. Further, as additional controls and for purposes of comparison, proteomic signatures were also obtained from intact cells and apoptotic bodies. The data obtained from LC-MS/MS, combined with database searches and protein assembly algorithms, allowed us to address significant differences in protein spectral counts and identify unique pathways that may be a component of the induction of the signature inflammatory cytokine response against BECs, including the Notch signaling pathway, interleukin (IL)8, IL6, CXCR2, and integrin signaling. Indeed, there are 11 proteins that localize specifically to apoptotic bodies of HiBEC and eight proteins that were specifically absent in HiBEC apoptotic bodies.
conclusionProteomic analysis of BECs from PBC liver compared to normal liver are significantly different, suggesting that an immunological attack affects the repertoire of proteins expressed and that such cells should be thought of as living in an environment undergoing continuous selection secondary to an innate and adaptive immune response, reflecting an almost "Darwinian" bias.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.