ArticleThe Journal of biological chemistry2014
Identification of the flagellin glycosylation system in Burkholderia cenocepacia and the contribution of glycosylated flagellin to evasion of human innate immune responses.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 64 citations in OpenAlex.
- Comparative genomic and phenotypic analysis ofmSphere · 2026Article
- Flagellar glycosylation with pseudaminic acids is widespread in the genus Clostridium.BMC microbiology · 2026Article
- Toll-like receptors in fish: evolution, structure, immune signaling, and translational prospects for adjuvant-based vaccine design in aquaculture.Frontiers in immunology · 2026Review
- Commensal bacterial glycosylation at the interface of host-bacteria interactions.Gut microbes · 2025Review
- Review
- Review
- The role of the glycome in symbiotic host-microbe interactions.Glycobiology · 2023Review
- Advances in protein glycosylation and its role in tissue repair and regeneration.Glycoconjugate journal · 2023Review
- Intervention Strategies to ControlMicroorganisms · 2023Review
- Host control and the evolution of cooperation in host microbiomes.Nature communications · 2022Article
- Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activateProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Defeated by the nines: nine extracellular strategies to avoid microbe-associated molecular patterns recognition in plants.The Plant cell · 2021Article
- Article
- Adaptation of the Gut Microbiota to Modern Dietary Sugars and Sweeteners.Advances in nutrition (Bethesda, Md.) · 2020Review
- Burkholderia cenocepacia-host cell contact controls the transcription activity of the trimeric autotransporter adhesin BCAM2418 gene.MicrobiologyOpen · 2020Article
- Phylogenetic Distribution, Ultrastructure, and Function of Bacterial Flagellar Sheaths.Biomolecules · 2020Review
- Protein post-translational modifications in bacteria.Nature reviews. Microbiology · 2019Review
- Glycointeractions in bacterial pathogenesis.Nature reviews. Microbiology · 2018Review
- A ten gene-containing genomic island determines flagellin glycosylation: implication for its regulatory role in motility and virulence of Xanthomonas oryzae pv. oryzae.Molecular plant pathology · 2018Article
- Genetic Determinants Associated WithFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia cenocepacia is an opportunistic pathogen threatening patients with cystic fibrosis. Flagella are required for biofilm formation, as well as adhesion to and invasion of epithelial cells. Recognition of flagellin via the Toll-like receptor 5 (TLR5) contributes to exacerbate B. cenocepacia-induced lung epithelial inflammatory responses. In this study, we report that B. cenocepacia flagellin is glycosylated on at least 10 different sites with a single sugar, 4,6-dideoxy-4-(3-hydroxybutanoylamino)-D-glucose. We have identified key genes that are required for flagellin glycosylation, including a predicted glycosyltransferase gene that is linked to the flagellin biosynthesis cluster and a putative acetyltransferase gene located within the O-antigen lipopolysaccharide cluster. Another O-antigen cluster gene, rmlB, which is required for flagellin glycan and O-antigen biosynthesis, was essential for bacterial viability, uncovering a novel target against Burkholderia infections. Using glycosylated and nonglycosylated purified flagellin and a cell reporter system to assess TLR5-mediated responses, we also show that the presence of glycan in flagellin significantly impairs the inflammatory response of epithelial cells. We therefore suggest that flagellin glycosylation reduces recognition of flagellin by host TLR5, providing an evasive strategy to infecting bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.