ArticleThe Journal of biological chemistry2014
Sialyl-lactotetra, a novel cell surface marker of undifferentiated human pluripotent stem cells.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Integrated Multi-Omics Analysis Uncovers Molecular Dysregulation in Glucosylceramide Synthase-Deficient Human Induced Pluripotent Stem Cells.Molecular & cellular proteomics : MCP · 2026Article
- Glycosphingolipids in human abdominal leiomyosarcoma and liposarcoma.The Journal of biological chemistry · 2026Article
- Glycan Markers of Pluripotent Stem Cells.Advances in experimental medicine and biology · 2026Review
- Article
- Endogenous ROS production in early differentiation state suppresses endoderm differentiation via transient FOXC1 expression.Cell death discovery · 2022Article
- Characterization of Human Medullary Thyroid Carcinoma Glycosphingolipids Identifies Potential Cancer Markers.International journal of molecular sciences · 2021Article
- Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands.International journal of molecular sciences · 2021Article
- Colonization factor CS30 from enterotoxigenicVirulence · 2020Article
- Characterization of glycosphingolipids from gastrointestinal stromal tumours.Scientific reports · 2020Article
- Review
- Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization.Biomolecules · 2020Article
- Evaluation of Sialyl-Lactotetra as a Marker for Epithelial Ovarian Tumors.Frontiers in oncology · 2020Article
- Glycan Markers of Human Stem Cells Assigned with Beam Search Arrays.Molecular & cellular proteomics : MCP · 2019Article
- Helicobacter pylori SabA binding gangliosides of human stomach.Virulence · 2018Article
- Analytical Scheme Leading to Integrated High-Sensitivity Profiling of Glycosphingolipids Together with N- and O-Glycans from One Sample.Journal of the American Society for Mass Spectrometry · 2018Article
- Glycosphingolipids of human embryonic stem cells.Glycoconjugate journal · 2017Review
- Glycosphingolipid dynamics in human embryonic stem cell and cancer: their characterization and biomedical implications.Glycoconjugate journal · 2017Review
- Glycomics of human embryonic stem cells and human induced pluripotent stem cells.Glycoconjugate journal · 2017Review
- HLA and Histo-Blood Group Antigen Expression in Human Pluripotent Stem Cells and their Derivatives.Scientific reports · 2017Article
- Glycomics of human embryonic stem cells and human induced pluripotent stem cells.Glycoconjugate journal · 2016Review
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell surface glycoconjugates are used as markers for undifferentiated pluripotent stem cells. Here, antibody binding and mass spectrometry characterization of acid glycosphingolipids isolated from a large number (1 × 10(9) cells) of human embryonic stem cell (hESC) lines allowed identification of several novel acid glycosphingolipids, like the gangliosides sialyl-lactotetraosylceramide and sialyl-globotetraosylceramide, and the sulfated glycosphingolipids sulfatide, sulf-lactosylceramide, and sulf-globopentaosylceramide. A high cell surface expression of sialyl-lactotetra on hESC and human induced pluripotent stem cells (hiPSC) was demonstrated by flow cytometry, immunohistochemistry, and electron microscopy, whereas sulfated glycosphingolipids were only found in intracellular compartments. Immunohistochemistry showed distinct cell surface anti-sialyl-lactotetra staining on all seven hESC lines and three hiPSC lines analyzed, whereas no staining of hESC-derived hepatocyte-like or cardiomyocyte-like cells was obtained. Upon differentiation of hiPSC into hepatocyte-like cells, the sialyl-lactotetra epitope was rapidly down-regulated and not detectable after 14 days. These findings identify sialyl-lactotetra as a promising marker of undifferentiated human pluripotent stem cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.