ArticlePloS one2014
Evaluation of reference genes for gene expression analysis using quantitative RT-PCR in Azospirillum brasilense.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
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- ι-Carrageenan catabolism is initiated by key sulfatases in the marine bacteriumApplied and environmental microbiology · 2024Article
- Genetic and phenotypic ofIranian journal of microbiology · 2024Article
- Selection and validation of reference genes suitable for gene expression analysis by Reverse Transcription Quantitative real-time PCR in Acinetobacter baumannii.Scientific reports · 2024Article
- Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Tissues from Bumble Bees (International journal of molecular sciences · 2022Article
- Identification and Validation of Reference Genes for Expression Analysis in Nitrogen-Fixing Bacteria under Environmental Stress.Life (Basel, Switzerland) · 2022Article
- A comparative study on the genomes, transcriptomes, and metabolic properties ofEngineering microbiology · 2022Article
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- Selection and stability validation of reference gene candidates for transcriptional analysis in Rousettus aegyptiacus.Scientific reports · 2021Article
- Engineering Posttranslational Regulation of Glutamine Synthetase for Controllable Ammonia Production in the Plant Symbiont Azospirillum brasilense.Applied and environmental microbiology · 2021Article
- ThemSystems · 2021Article
- Expression and function of the cdgD gene, encoding a CHASE-PAS-DGC-EAL domain protein, in Azospirillum brasilense.Scientific reports · 2021Article
- Deep (Meta)genomics and (Meta)transcriptome Analyses of Fungal and Bacteria Consortia From Aircraft Tanks and Kerosene Identify Key Genes in Fuel and Tank Corrosion.Frontiers in microbiology · 2021Article
- Phenotypic and Transcriptomic Analyses of Seven Clinical Stenotrophomonas maltophilia Isolates Identify a Small Set of Shared and Commonly Regulated Genes Involved in the Biofilm Lifestyle.Applied and environmental microbiology · 2020Article
- Reference gene selection for qRT-PCR analysis of season- and tissue-specific gene expression profiles in the honey bee Apis mellifera.Scientific reports · 2020Article
- Article
- mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression.Molecular biology reports · 2019Article
- Reference genes for real-time RT-PCR expression studies in an Antarctic Pseudomonas exposed to different temperature conditions.Extremophiles : life under extreme conditions · 2019Article
- DisarmingFrontiers in microbiology · 2019Article
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Azospirillum brasilense is a nitrogen fixing bacterium that has been shown to have various beneficial effects on plant growth and yield. Under normal conditions A. brasilense exists in a motile flagellated form, which, under starvation or stress conditions, can undergo differentiation into an encapsulated, cyst-like form. Quantitative RT-PCR can be used to analyse changes in gene expression during this differentiation process. The accuracy of quantification of mRNA levels by qRT-PCR relies on the normalisation of data against stably expressed reference genes. No suitable set of reference genes has yet been described for A. brasilense. Here we evaluated the expression of ten candidate reference genes (16S rRNA, gapB, glyA, gyrA, proC, pykA, recA, recF, rpoD, and tpiA) in wild-type and mutant A. brasilense strains under different culture conditions, including conditions that induce differentiation. Analysis with the software programs BestKeeper, NormFinder and GeNorm indicated that gyrA, glyA and recA are the most stably expressed reference genes in A. brasilense. The results also suggested that the use of two reference genes (gyrA and glyA) is sufficient for effective normalisation of qRT-PCR data.
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