Evidence map›Paper›PMID 24840617›Full record

ArticleBiomaterials2014

The effects of confinement on neuronal growth cone morphology and velocity.

Michael S Smirnov, Katelyn A Cabral, Herbert M Geller, Jeffrey S Urbach

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 11 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Michael S SmirnovInterdisciplinary Program in Neuroscience, Georgetown University, Washington, DC 20057, USA; Department of Physics and the Institute for Soft Matter Synthesis and Metrology, Georgetown University, 320 Regents Hall, Washington, DC 20057, USA.
Katelyn A CabralDevelopmental Neurobiology Section, Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Herbert M GellerDevelopmental Neurobiology Section, Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Jeffrey S UrbachDepartment of Physics and the Institute for Soft Matter Synthesis and Metrology, Georgetown University, 320 Regents Hall, Washington, DC 20057, USA. Electronic address: urbach@physics.georgetown.edu.
Georgetown University · USNational Institutes of Health · US

Funding

NHLBI Animal Program DivisionZIGHL006020 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI CLARK, TANNIA · 2009 to 2025
$47.3M
Light Microscopy CoreZICHL006019 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI COMBS, CHRISTIAN · 2009 to 2025
$29.3M
Signaling by Chondroitin Sulfate ProteoglycansZIAHL006021 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI GELLER, HERBERT M. · 2009 to 2022
$12.3M
Predoctoral Training in Intergrative NeuroscienceT32NS041231 · NINDS · GEORGETOWN UNIVERSITY · PI Ludise Malkova, Ashley S VanMeter · 2001 to 2026
$6.8M
Mechanisms of axonal pathfinding in three dimensional matricesR01NS064250 · NINDS · GEORGETOWN UNIVERSITY · PI URBACH, JEFFREY S. · 2009 to 2013
$1.6M
Intramural NIH HHS ZIA HL006021NHLBI NIH HHS 1 ZIA HL006021NINDS NIH HHS 1R01NS064250NINDS NIH HHS R01 NS064250NINDS NIH HHS T32 NS041231
6 · The paper itself

Abstract

Optimizing growth cone guidance through the use of patterned substrates is important for designing regenerative substrates to aid in recovery from neuronal injury. Using laser ablation, we designed micron-scale patterns capable of confining dissociated mouse cerebellar granule neuron growth cones to channels of different widths ranging from 1.5 to 12 μm. Growth cone dynamics in these channels were observed using time-lapse microscopy. Growth cone area was decreased in channels between 1.5 and 6 μm as compared to that in 12 μm and unpatterned substrates. Growth cone aspect ratio was also affected as narrower channels forced growth cones into a narrow, elongated shape. There was no difference in the overall rate of growth cone advance in uniform channels between 1.5 and 12 μm as compared to growth on unpatterned substrates. The percentage of time growth cones advanced, paused, and retracted was also similar. However, growth cones did respond to changes in confinement: growth cones in narrow lanes rapidly sped up when encountering a wide region and then slowed down as they entered another narrow region. Our results suggest that the rate of neurite extension is not affected by the degree of confinement, but does respond to changes in confinement.

Indexed as

AnimalsCells, CulturedCoated Materials, BiocompatibleGrowth ConesImage Processing, Computer-AssistedLamininMiceMice, Inbred C57BLMicroscopy, Atomic ForceMicroscopy, Electron, ScanningNerve RegenerationNeurogenesisNeuronsCoated Materials, BiocompatibleLamininLamininLaser ablationNerve regenerationNeural cellPolyvinylalcohol

Identifiers

PMID24840617
PMCPMC4075440
OpenAlexW1999359186

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.