ArticlePloS one2014
A causal relation between bioluminescence and oxygen to quantify the cell niche.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Targeted intracellular delivery of molecular cargo to hypoxic human breast cancer stem cells.bioRxiv : the preprint server for biology · 2024Article
- A New Perspective on Stroke Research: Unraveling the Role of Brain Oxygen Dynamics in Stroke Pathophysiology.Aging and disease · 2024Review
- Bioluminescence as a functional tool for visualizing and controlling neuronal activityNeurophotonics · 2024Article
- Oxygen imaging of hypoxic pockets in the mouse cerebral cortex.Science (New York, N.Y.) · 2024Article
- Practical Guidance for Developing Small-Molecule Optical Probes for In Vivo Imaging.Molecular imaging and biology · 2023Review
- Physiological Responses of the FireflyFrontiers in physiology · 2022Article
- A Genetically Encoded Bioluminescence Intracellular Nanosensor for Androgen Receptor Activation Monitoring in 3D Cell Models.Sensors (Basel, Switzerland) · 2021Article
- Turn-on chemiluminescence probes and dual-amplification of signal for detection of amyloid beta species in vivo.Nature communications · 2020Article
- Article
- Modular low-light microscope for imaging cellular bioluminescence and radioluminescence.Nature protocols · 2017Article
- Modular platform for low-light microscopy.Biomedical optics express · 2015Article
- Correction: A Causal Relation between Bioluminescence and Oxygen to Quantify the Cell Niche.PloS one · 2015Article
Corrections and comments
- Erratum issuedCorrection: A Causal Relation between Bioluminescence and Oxygen to Quantify the Cell Niche.2015 · Vande Velde, Greetje [added]
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bioluminescence imaging assays have become a widely integrated technique to quantify effectiveness of cell-based therapies by monitoring fate and survival of transplanted cells. To date these assays are still largely qualitative and often erroneous due to the complexity and dynamics of local micro-environments (niches) in which the cells reside. Here, we report, using a combined experimental and computational approach, on oxygen that besides being a critical niche component responsible for cellular energy metabolism and cell-fate commitment, also serves a primary role in regulating bioluminescent light kinetics. We demonstrate the potential of an oxygen dependent Michaelis-Menten relation in quantifying intrinsic bioluminescence intensities by resolving cell-associated oxygen gradients from bioluminescent light that is emitted from three-dimensional (3D) cell-seeded hydrogels. Furthermore, the experimental and computational data indicate a strong causal relation of oxygen concentration with emitted bioluminescence intensities. Altogether our approach demonstrates the importance of oxygen to evolve towards quantitative bioluminescence and holds great potential for future microscale measurement of oxygen tension in an easily accessible manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.