ArticleJournal of diabetes research2014
The visualization of biofilms in chronic diabetic foot wounds using routine diagnostic microscopy methods.
Article in Journal of diabetes research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 67 citations in OpenAlex.
- Article
- Characteristic of Virulence and Parameters of Mixed Biofilm Formed by Carbapenem-ResistantPathogens (Basel, Switzerland) · 2025Article
- A Scoping Review to Identify Clinical Signs, Symptoms and Biomarkers Reported in the Literature to Be Indicative of Biofilm in Chronic Wounds.International wound journal · 2025Article
- The Performance of Gram-Staining in Tailoring the Empirical Antibiotic Choice in Operated Diabetic Foot Infections.Journal of clinical medicine · 2025Article
- Microbial extracellular polymeric substances in the environment, technology and medicine.Nature reviews. Microbiology · 2025Review
- A novel dual-staining method for cost-effective visualization and differentiation of microbial biofilms.Scientific reports · 2024Article
- A survey of multiple candidate probiotic bacteria reveals specificity in the ability to modify the effects of key wound pathogens.Microbiology spectrum · 2024Article
- Loop-Mediated Isothermal Amplification of DNA (LAMP) as an Alternative Method for Determining Bacteria in Wound Infections.International journal of molecular sciences · 2023Review
- Ciprofloxacin enhances the biofilm formation ofFrontiers in microbiology · 2023Article
- Article
- Article
- Biofilms: Formation, Research Models, Potential Targets, and Methods for Prevention and Treatment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Possible clinical implications and future directions of managing bacterial biofilms in cutaneous leishmaniasis wounds.Tropical medicine and health · 2022Article
- Novel Diagnostic Technologies and Therapeutic Approaches Targeting Chronic Wound Biofilms and Microbiota.Current dermatology reports · 2022Article
- Biofilm Survival Strategies in Chronic Wounds.Microorganisms · 2022Review
- Biofilms: Formation, drug resistance and alternatives to conventional approaches.AIMS microbiology · 2022Review
- Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds.Scientific reports · 2021Article
- Detection of bacterial fluorescence from in vivo wound biofilms using a point-of-care fluorescence imaging device.International wound journal · 2021Article
- Diagnostics for Wound Infections.Advances in wound care · 2021Review
- Analysis of Biofilm Formation on the Surface of Organic Mung Bean Seeds, Sprouts and in the Germination Environment.Foods (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diabetic foot wounds are commonly colonised by taxonomically diverse microbial communities and may additionally be infected with specific pathogens. Since biofilms are demonstrably less susceptible to antimicrobial agents than are planktonic bacteria, and may be present in chronic wounds, there is increasing interest in their aetiological role. In the current investigation, the presence of structured microbial assemblages in chronic diabetic foot wounds is demonstrated using several visualization methods. Debridement samples, collected from the foot wounds of diabetic patients, were histologically sectioned and examined using bright-field, fluorescence, and environmental scanning electron microscopy and assessed by quantitative differential viable counting. All samples (n = 26) harboured bioburdens in excess of 5 log₁₀ CFU/g. Microcolonies were identified in 4/4 samples by all three microscopy methods, although bright-field and fluorescence microscopy were more effective at highlighting putative biofilm morphology than ESEM. Results in this pilot study indicate that bacterial microcolonies and putative biofilm matrix can be visualized in chronic wounds using fluorescence microscopy and ESEM, but also using the simple Gram stain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.